Neuromelanin triggers dendritic cell (DC) maturation. Flow cytometric analysis of DC maturation by staining with anti-CD86/anti-MHCII antibodies. A: medium-treated DCs (untreated). B: DAM-treated DCs. C: NM-treated DCs. D: positive control (LPS-treated DCs). A-D: representative experiments from triplicates. E: Illustration of the amount of mature DCs from triplicates (data as mean ± S.E.M.; statistical analysis with one-way ANOVA using Bonferroni multiple comparison as post test; n.s. = non specific (p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001).