CGRP and MBP expression in rat C1. (A) CGRP expression in laminae I and II (arrow) in rat C1. (B) Transverse CGRP positive fibers (arrows) and CGRP positive neurons (arrow heads) are found close to the central canal. (C, D) Double-staining of CGRP (red) and MBP (green). Arrows point at CGRP positive areas negative for MBP. Nuclei staining (DAPI, blue) is used in the merged pictures.