CLR/RAMP1 and CGRP double-staining in human C1. (A) Expression of RAMP1 and CLR in human C1. The receptor components are found within laminae I and II. (B) Double-staining of CGRP (red) and RAMP1 (green) shows that CGRP positive fibers (arrows) within laminae I and II do not co-localize with RAMP1. Asterisks point at autofluorescent lipofuscin.