Comparison of automated analysis with NeuriteQuant to manual analysis and existing software. a) P19 cells were cultured with or without transfection of the neurogenic transcription factor NeuroD2. Neurite length was measured either by manual tracing using the semi-automated NeuronJ program , the NeuriteQuant package, the method by Wu et al.  or the NeuriteIQ software. b) Box and Whisker graphs from quantitative measurements of neurite length from 8 microscopic fields per condition. c) Corresponding automated and manual analyses of neurite length of identical images are symbolized by a connecting line.