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Figure 1 | BMC Neuroscience

Figure 1

From: An adeno-associated viral vector transduces the rat hypothalamus and amygdala more efficient than a lentiviral vector

Figure 1

In vitro testing of the antibodies against GFP and dsred. A: schematic overview of AAV vector used. B: schematic overview of LV vector used. C: shows endogenous dsRed fluorescence (red) in 293T cells transfected with CMV-dsRed. C':shows immunostaining for dsRed (green) in these cells. C'': shows the co-localization of the immunostained and endogenous fluorescence (yellow). D: shows endogenous fluorescence (green) in 293T cells transfected with CMV-GFP. D': shows immunostaining for GFP (red) and D'' shows the overlay of D and D' (green nucleus with red cytoplasma). E: 293T cells co-transfected with CMV-GFP and CMV-dsred were immunostained for GFP (red (E)) and dsRed (green (E')). These stainings overlap (yellow (E'')). Scalebar is 50 μm.

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