Figure 4
From: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates
Homogeneous assays in aNS-1 derived neurons. (A): 3DIV aNS-1 differentiated cells were exposed to H2O2 or STS in 96-well microplates for 24 h. Caspase 3/7 activation, normalized on vitality data as assessed by MTT assay, is reported. Results from one experiment performed in triplicate are shown. Three independent experiments give rise to overlapping results. (B): 21DIV aNS-1 differentiated cells were exposed to Forskolin 250 μM for 15' and cAMP-Glo Assay™ was then performed on 19 well from a 96-well microplate. Row data dispersion around mean value (full line) is reported; upper and lower groups represent untreated control and treated well data respectively; dot lines represent 2SD from mean values: letters in x axes identify each individual well. (C): 21DIV aNS-1 differentiated cells were exposed to adenosine receptor agonist/antagonist for 1 h. Forskolin for 15' was used as positive control. Graph shows cAMP levels modulation, average value from three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01. H2O2: Hydrogen Peroxide; cps, counts per second; STS: staurosporine.