Figure 4

Transcriptional activity of the mouse Dcx upstream regulatory sequences in embryonic stem cells during differentiation. Every second day, cells were co-transfected with the indicated Dcx promoter construct or the promoterless control (CTL). Normalized luciferase activity corresponds to the luciferase activity (expressed in RLU) of each transfection (determined 48 hours later) normalized to the corresponding internal control, β-Galactosidase activity (expressed in OD at 420 nm) (see materials and methods). Each value represents mean ± SEM of at least three independent transfection experiments, each performed in triplicate.