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Table 1 Sequence of PCR cloning primers

From: Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs

Construct

Forward primer1

Reverse Primer 1

pMayven-EGFP

(Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'

pΔSH3

(Bgl II) 5'-AGATCT CCCACCATGTGCACAAAGCAGGGTCATCAG-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pΔFYN

(Bgl II) 5'-AGATCT CCCACCATGAACGAACTAAGAAGTCAA-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pΔBTB/POZ

(Bgl II) 5'-AGATCT CCCACCATGGTCCTCCTCCCAGCAGCTGGC-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pΔBACK

(Bgl II) 5'-AGATCT CCCACCATGAACCTTCCCAAATTGATG-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pΔKELCH

(Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †

(Hind III) 5'-AAGCCT TTTGGGAAGGTTCATGGGTGT-3'

pΔKELCH-456

(Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †

(Hind III) 5'-AAGCCT ACCTCCAACAACGCCAACGCC-3'

pΔKELCH-6

(Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †

(Hind III) 5'-AAGCCT ACCATTAACTGCGCAGACACC-3'

pΔPPL

(Hind III) 5'-AGATCT CCCACCATGCCTCCCGCATGCACAAAGCAG-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pQA

(Hind III) 5'-AGATCT CCCACC ATGGAGACGCAGCCGCTGCCTGCCGCA-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pDK

(Hind III)5'-AGATCT CCCACCATGGACAAGCCGCCGCTGCCTCCCGCA-3'

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †

pEGFP-Mayven

(Xho I) 5'-CTCGAG ATGGAGACGCCGCCGCTGCCT-3'.

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'

pEGFP-ΔFYN

(Xho I) 5'-CTCGAG-ATGAACGAACTAAGAAGTCAA-3'.

(Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'

pMayven-HA

(Hind III) 5'-AGCTT CCCACCATGGAGACGCCGCCGCTGCCT-3'

(Kpn I) 5'-GGTACC TAATGGTTTATCAATAACTGT-3'.

  1. 1 Restriction enzyme sites are italicised and corresponding sequences are in bold. The artificial Kozak motif is underlined.
  2. † Common primers used in the construction of the three sets of Mayven mutants.