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Table 1 Sequence of PCR cloning primers

From: Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs

Construct Forward primer1 Reverse Primer 1
pMayven-EGFP (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pΔSH3 (Bgl II) 5'-AGATCT CCCACCATGTGCACAAAGCAGGGTCATCAG-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pΔFYN (Bgl II) 5'-AGATCT CCCACCATGAACGAACTAAGAAGTCAA-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pΔBTB/POZ (Bgl II) 5'-AGATCT CCCACCATGGTCCTCCTCCCAGCAGCTGGC-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pΔBACK (Bgl II) 5'-AGATCT CCCACCATGAACCTTCCCAAATTGATG-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pΔKELCH (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' (Hind III) 5'-AAGCCT TTTGGGAAGGTTCATGGGTGT-3'
pΔKELCH-456 (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' (Hind III) 5'-AAGCCT ACCTCCAACAACGCCAACGCC-3'
pΔKELCH-6 (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' (Hind III) 5'-AAGCCT ACCATTAACTGCGCAGACACC-3'
pΔPPL (Hind III) 5'-AGATCT CCCACCATGCCTCCCGCATGCACAAAGCAG-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pQA (Hind III) 5'-AGATCT CCCACC ATGGAGACGCAGCCGCTGCCTGCCGCA-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pDK (Hind III)5'-AGATCT CCCACCATGGACAAGCCGCCGCTGCCTCCCGCA-3' (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pEGFP-Mayven (Xho I) 5'-CTCGAG ATGGAGACGCCGCCGCTGCCT-3'. (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pEGFP-ΔFYN (Xho I) 5'-CTCGAG-ATGAACGAACTAAGAAGTCAA-3'. (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3'
pMayven-HA (Hind III) 5'-AGCTT CCCACCATGGAGACGCCGCCGCTGCCT-3' (Kpn I) 5'-GGTACC TAATGGTTTATCAATAACTGT-3'.
  1. 1 Restriction enzyme sites are italicised and corresponding sequences are in bold. The artificial Kozak motif is underlined.
  2. Common primers used in the construction of the three sets of Mayven mutants.