From: Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs
Construct | Forward primer1 | Reverse Primer 1 |
---|---|---|
pMayven-EGFP | (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' |
pΔSH3 | (Bgl II) 5'-AGATCT CCCACCATGTGCACAAAGCAGGGTCATCAG-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pΔFYN | (Bgl II) 5'-AGATCT CCCACCATGAACGAACTAAGAAGTCAA-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pΔBTB/POZ | (Bgl II) 5'-AGATCT CCCACCATGGTCCTCCTCCCAGCAGCTGGC-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pΔBACK | (Bgl II) 5'-AGATCT CCCACCATGAACCTTCCCAAATTGATG-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pΔKELCH | (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †| (Hind III) 5'-AAGCCT TTTGGGAAGGTTCATGGGTGT-3' |
pΔKELCH-456 | (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †| (Hind III) 5'-AAGCCT ACCTCCAACAACGCCAACGCC-3' |
pΔKELCH-6 | (Bgl II)5'-AGATCT CCCACCATGGAGACGCCGCCGCTGCCT-3' †| (Hind III) 5'-AAGCCT ACCATTAACTGCGCAGACACC-3' |
pΔPPL | (Hind III) 5'-AGATCT CCCACCATGCCTCCCGCATGCACAAAGCAG-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pQA | (Hind III) 5'-AGATCT CCCACC ATGGAGACGCAGCCGCTGCCTGCCGCA-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pDK | (Hind III)5'-AGATCT CCCACCATGGACAAGCCGCCGCTGCCTCCCGCA-3' | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' †|
pEGFP-Mayven | (Xho I) 5'-CTCGAG ATGGAGACGCCGCCGCTGCCT-3'. | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' |
pEGFP-ΔFYN | (Xho I) 5'-CTCGAG-ATGAACGAACTAAGAAGTCAA-3'. | (Hind III) 5'-AAGCTT TAATGGTTTATCAATAACTGT-3' |
pMayven-HA | (Hind III) 5'-AGCTT CCCACCATGGAGACGCCGCCGCTGCCT-3' | (Kpn I) 5'-GGTACC TAATGGTTTATCAATAACTGT-3'. |