Figure 6

TRPC2 cell-surface biotinylation. Representative Western blot of TRPC2-biotinylated protein following 48 hours after transfection of indicated cDNAs in HEK293s. (A) (Top panel) Biotinylated protein products (see methods) were precipitated with streptavidin agarose beads, subjected to SDS-PAGE, and then probed with α-c-myc (1:400) to visualize myc-Kv1.3 expression (far left lane) versus that of mycTRPC2 expression under various transfection conditions. (Bottom panel) Lysate input probed with α-β-actin. (B) Histogram plot of the mean ± s.e.m. pixel immunodensity normalized to that of the TRP alone condition. Transfection sample size noted. *= Mean pixel density is statistically-different from channel only transfection; Dunnett's post-hoc test, p ≤ 0.05. (C) Ratio of the expression levels of mycTRPC2 and the internal standard, myc protein. Values are normalized against the channel only condition.