Figure 3

Glycine immunocytochemistry at the junction of the 'tail' and 'head' region of Ciona larvae. (A) Fluorescent image of the junction of tail and 'head' showing the location of glycine positive elements (red). Note the two clearly discernable glycine-positive cells (small arrows) in the nerve cord. (B) Brightfield image of the same area. (C) Fluorescent image of a similar area as in A showing dual staining of glycine positive material (red) and nuclei (green). In this case, at least four glycine positive elements may be identified as being cellular (yellow co-label). Large arrows indicate the junction between the 'head' and tail of the larva. Scale bars = 10 μm.