Androgen receptor mRNA expression in the saccule of male and female Astatotilapia burtoni. (A) mRNA levels of ARα differed among brooding, recovering and gravid females, but not between dominant and subordinate males. Inset: ARα mRNA levels in the brain were higher in gravid females compared to both recovering and brooding animals. (B) mRNA levels of ARβ also differed among all three female reproductive states, but did not differ among subordinate and dominant males. Data are plotted as relative mRNA levels (mean ± SE) referenced to the geometric mean of two housekeeping genes (18s and G3PDH). Bars with different letters represent significant differences (p < 0.05) and sample sizes are indicated within each bar on the bottom graphs.