Neurogenesis and gliogenesis from adult human NSCs. A, The percentage of GFAP+, MAP2ab+, β-tubulin III and GALC+ cells after differentiation for 10 to 14 days in KO/DMEM or CSF. Results are mean values ± S.E.M from at least three independent experiments. * indicates P < 0.05 compared to each corresponding value in CSF. B, Representative microphotographs demonstrating the effects of CSF on neuronal and glial differentiation of ahNSCs 10 to 14 days after initiation of the differentiation process. Cells were stained with anti-GFAP, anti-β-tubulin III and anti-GALC. Nuclei were counterstained with DAPI. Scale bar = 50 μm.