Mecp2 mRNA levels are similar in WT and A140V mouse cerebellum by qRT-PCR. (3A) Quantitative real time PCR (qRT-PCR) reactions were performed using PCR assays for Mecp2 and Gapdh. qRT-PCR data graphs (Upper Panels) and melting curves (Lower Panels) for Mecp2 and Gapdh are shown. (3B) A table of Ct (cycle threshold) values is shown including mean and standard deviation (in parentheses) and ΔCt used in the calculation of fold change (F) in mRNA levels. F (Mecp2:Gapdh) = 0.91.