Figure 6
From: Signaling involved in neurite outgrowth of postnatally born subventricular zone neurons in vitro
Analysis of small GTPase involvement in lamellipodia formation of SVZ/RMS-derived neurons. Lamellipodia outgrowth analysis in control (A) cultures and in cultures after C. difficile Toxin A and Rac1 inhibitor treatment (B) and (C), respectively. Arrowheads in insets mark large lamellipodia. Cultured cells were treated with inhibitors and the amount of neurons having large lamellipodia on cell body and major cell processes was calculated at DIV4. (D) While Rac1 inhibitor significantly decreased the number of neurons with large lamellipodia, Toxin A increased it (* - p < 0.001). Abbreviations: Phal-TRITC - phalloidin-TRITC complex to label F-actin, Ra - Rac1 inhibitor, TA - Rho GTPases inhibitor Toxin A of C. difficile, Tuj1 - tubulin beta III to label immature neurons. Scale bars are 20 μm.