Co-localization of EGFP and neuronal subtype-specific markers. Twenty-nine days after the last TAM administration, the EGFP-expressing cells (green) were examined for the expression of specific markers (red). (a) co-localization with the dopaminergic marker tyrosine hydroxylase (TH) in the pGI, (b) co-localization with the GABAergic marker glutamate decarboxylase 65 (GAD65) in the pGI and (c) co-localization with the glutamatergic marker vesicular glutamate transporter 2 (VGLUT2) in the DG. (d) Co-localization with DG mature granule cell marker calbindin-D28K, (e) co-localization with the early DG granule cell marker calretinin. Arrow points to a cell expressing high levels of calretinin, but no EGFP, (f) absence of co-localization with parvalbumin in the DG. Scale bar in (e) = 33 μm.