Profiles of gene expression during neural stem cell-mediated neural lineage elaboration. (A) Immunofluorescence micrographs of cellular expression patterns of lineage markers and bHLH transcription factors during progressive GABAergic neuronal and OL lineage elaboration from N/OPs: NSCs are labeled with nestin (TRITC) only, while N/OPs (white arrows) express both Olig2 (FITC, green arrow head) and Mash 1 (TRITC, red arrow head). GABANs expressed GABA (FITC). OLPs are stained with O4 (FITC), while PMOs and MYOs are identified by the expression of GC/O1 (FITC) and MBP (FITC), respectively. (B) Schematic flowchart illustrating the stages of oligodendrocyte and GABAergic neuron lineages analyzed within this study. (C) Tree-plot showing expressed ncRNAs clustered according to differential expression during microarray analysis. (D) Expression of mRNA marker genes with well characterized roles in GABAergic neuronal and progressive stages of OL lineage elaboration as determined by microarray.