Figure 2

Localization of transplanted cells and in situ proliferation at the infarct border in experimental model. (A-C) are images of coronal cerebral slices showing the impact of occlusion 6 hours post-occlusion, with the infarct appearing as pale area marked with broken line. Hematoxylin and eosin (H.E.) stain illustrates loss of relatively large cells and infiltration of small cells at the border of the infarct (D). Paired cellular profiles are seen peripheral to the infarct border or the infarct penumbra (E). At low magnification, a large number of transplanted bone marrow cells pre-labeled by PKH26 (red fluorescence) are present in the infarct penumbra 7 days post lesion (F). The labeled cells are small and may occur in cluster (G). No fluorescent cells exist in the cerebral cortex of mice received vehicle infusion (H). In situ cell division reflected by immunoreactivity of proliferating cell nuclear antigen (PCNA) occurs predominantly at the infarct border (I) and penumbra (J), appearing as brown immunoreactive nuclei in hematoxylin counter-stained section.