Figure 5

Light microscopy analysis of BrdU-positive cells in p75^NTR (+/+) and (-/-) dentate gyrus. Coronal sections of the dentate gyrus harvested from p75 ^NTR (+/+) (B, D) and p75 ^NTR (-/-) (C, E) transgenic mice were examined. (A) Coronal schematic of anterior and posterior dentate gyrus (modified from [54]). In p75 ^NTR (+/+) tissues (B and D), BrdU-positive cells are found in a characteristic arrangement in the SGZ adjacent to the dentate gyrus in both anterior (B) and posterior (D) sections. Labeled cells are also present in the hilar regions (arrows). In p75 ^NTR (-/-) mice (C and E) an apparent decrease in the number of BrdU-positive cells was found in both anterior (C) and posterior (E) regions. Labeled cells are absent in the hilar regions in p75^NTR (-/-) (arrows in C and E). (F, G) Higher magnification of labeled SGZ cells demonstrates characteristic aggregation of progenitor cells in p75^NTR (+/+) sections with less aggregate apparent in (-/-) sections. Scale bar 25 μm. Abbreviations: ant: anterior; post: posterior; sgz: subgranular zone; DG: dentate gyrus. Scale bar: 100 μm.