Figure 6From: Distinct target cell-dependent forms of short-term plasticity of the central visceral afferent synapses of the ratDistribution of PPR of DMX neurons is related to the projection of the neuron. A, identification of and targeted recording from a DMX neuron that projected to the anterior gastric branches of the subdiaphragmic vagus nerve, onto which DiI was placed. Left, epifluorescent image of the DiI fluorescence in the DMX; middle, IR-DIC image of the same observation field with a recording pipette on a DiI-positive neuron; right, fluorescence image of Alexa Fluor 488 injected into the identified neuron via internal solution. B, locations of the DiI-positive recorded DMX neurons (filled squares, DMX neurons classified as type I; open circles, those classified as type II) and the position of the tip of the stimulation electrode (crosses). C, distribution of PPR values among the DMX neurons. Gray bars: distribution of non-identified DMX neurons recorded from rats without DiI pretreatment (the same as in Fig. 1C); open cityscape histogram, that of DiI-positive DMX neurons. Insets above show averaged EPSC traces from two DiI-positive DMX neurons representing type I (left) and type II (right). D, cumulative probability plot based on the histogram in C. The distribution of PPR values of DiI-positive DMX neurons and that of DMX neurons recorded in the slices from non-operated rats were significantly different (Kolmogorov-Smirnov test, p = 0.035).Back to article page