Hippocampal neurons in co-culture at 7 DIV. WT primary embryonic hippocampal neurons (MAP2, green) on top of a monolayer of WT primary cortical astrocytes (GFAP, red). Nuclei are stained with DAPI (blue). Scale bar represents 100 μm. Inset is an example of an isolated neuron that would be selected for morphometric analysis.