Figure 4

Quantitation of Synaptophysin Immunoreactivity. A shows a bar graph representation of synaptophysin immunoreactive presynaptic terminals in the molecular layer of the dentate gyrus of the hippocampus. The differences between 6D11 treated Tg mice (n = 10) and vehicle treated Tg mice (n = 8) are statistically significant by one-tailed t-test (p = 0.0267). B-E show representative sections immunostained with anti-synaptophysin antibody in the hippocampus at 10x magnification (B and C- Scale bar = 100 μm) and at 100x magnification (D and E- Scale bar = 10 μm) with the green box indicating the area of molecular layer magnified to the higher power. Images are of representative 6D11 treated Tg mice (B, D) and vehicle treated Tg mice (C, E).