Quantitative PCR showing differential expression of Prdx6 and NF-κB mRNA in RGCs with or without treatment with CoCl
or exposed to 1% O
. Total RNA was isolated and transcribed into cDNA. PCR was performed using specific primers as described in 'Methods' section. mRNA expression of each Prdx6 and NF-κB was adjusted to the mRNA copies of β-actin. Results indicate that mRNA expression level of Prdx6 was significantly increased after 24 h of exposure to different concentrations of CoCl2 (A; 50, 100 or 200 μM) with an increase of NF-κB mRNA. (B) CAT assay showing promoter activity of Prdx6 following hypoxia. Cells were transiently transfected with Prdx6-CAT (B & C). After 24 h of transfection, cells were either treated with cobalt chloride (50, 100 or 200 μM) (B) or exposed to 1% O2 (C; black bar). Transactivation assay was performed after 72 h of transfection. Results indicate that Prdx6 transcription (B and C) was up-regulated following cobalt chloride or 1% O2 exposure, respectively. *p < 0.05.