EphrinA5 protein expression in E12.5 embryonic mouse brain. EphrinA5 protein distribution is represented on coronal sections in a rostro-caudal sequence (panels a to g), and on sagittal sections in a latero-medial sequence (panels h to l). EphrinA5 protein was strongly expressed in the telencephalon (panels a, b, c, h, i) diencephalon (panels d, e, f, h, j, l) mesencephalon (panel k) and moderately in the metencephalon (panel g). Arrowheads in panel b, c and d indicate a lateral and ventral (high) to medial and dorsal (low) gradient in the lateral and medial ganglionic eminence, and preoptic differentiating zones respectively. In panel j and l, arrowheads show a ventral and rostral (high) to a dorsal and caudal (low) gradient of ephrinA5 protein in the ventral thalamus and hypothalamus respectively. Scale bar: panels a, c: 75 μm; panel b: 250 μm; panel d: 150 μm; panels e, f, g, h, i, l: 300 μm; panel j: 350 μm; panel k: 200 μm. Abbreviations: see list.