Figure 4
Antidepressant drug effects on CDP-diacylglycerol synthase activity in mouse cortex tissues. The enzymatic activity of CDP-diacylglycerol synthase (CDS) was measured in mouse cortical tissue preparations by determining the incorporation of radiolabeled cytidine derived from 5- [3H]CTP into CDP-diacylglycerol. Antidepressant agents were tested at concentrations ranging from 1 μM to 100 μM. Data from each assay were normalized against the control samples by calculating the percentages shown. Each bar is the mean ± SEM (N = 3 experiments). One-Way ANOVA analyses for each drug showed significant effects for imipramine (p < 0.001), maprotiline (p < 0.0001), and paroxetine (p < 0.01). Posthoc Dunnett tests showed that imipramine and maprotiline elicited significant effects at 3-100 μM concentrations while with paroxetine significant effects were evident at concentrations of 3-30 μM. *p < 0.05; ** P < 0.01; ***p < 0.001, compared to controls.