Figure 3
Effects of antidepressant agents on phosphatidylinositide synthesis in PC12 cells. PC12 cells were labeled with [3H]inositol and cells in different culture wells incubated with various concentrations of selected antidepressant drugs. After 3 h, accumulated [3H]phosphatidylinositides were determined and the data calculated as percentages relative to control. Each bar represents the mean ± SEM (N = 3 experiments). Each drug treatment increased the levels of [3H]phosphatidylinositides significantly and concentration-dependently (ANOVA, P < 0.0001 for each drug). Posthoc Dunnett tests showed statistically significant increases in phosphatidylinositides at 1-30 μM for imipramine and at 3-30 μM for maprotiline. *p < 0.05; ** P < 0.01; ***p < 0.001; compared to controls.