Figure 2

Effects of antidepressant agents on [³H]CDP-diacylglycerol synthesis in PC12 cells. PC12 cells were prelabeled with [³H]cytidine for 30 min and cells in different culture wells incubated with various concentrations of the antidepressant drugs imipramine, paroxetine or maprotiline. [³H]CDP-diacylglycerol was determined after 3 h. Data were calculated as percentages relative to the control incubations that received no drug treatment. Each bar represents the mean ± SEM (N = 3 experiments). The drug treatments increased [³H]CDP-diacylglycerol significantly (ANOVA, p < 0.0001 for each drug). Posthoc Dunnett tests showed statistically significant increases in CDP-diacylglycerol at 30 and 100 μM. *p < 0.05; ***p < 0.001, compared to controls.