Figure 2

Neuronal progenitors are located in the ventral telencephalon near the pial surface. Sagittal sections of E12 rat ventral telencephalon were immunostained with anti-nestin (blue), anti-PCNA (yellow), anti-CDw60/Jones (green) and anti-beta-tubulin III/Tuj1 (red). Tuj1 antibody was used in place of TnTx to provide a better visualization of neuronal cell bodies. Insets 1–3 in the top panel are enlarged in the bottom panels (1A-D, 2A-D, 3A-D) to show the anatomical distribution of the 4 major neural phenotypes, which were classified as follows: (a) Nestin⁺PCNA⁺Jones^-Tuj1^- neural stem/precursor cells, (b) Nestin⁺PCNA⁺Jones^-Tuj1⁺ neuronal progenitors and post-mitotic neurons, which are predominantly Nestin^-PCNA^-, (c) Nestin⁺PCNA⁺Jones⁺Tuj1^- neuroglial progenitors and (d) Nestin⁺PCNA⁺Jones⁺Tuj1⁺ neuronal progenitors, The majority of Jones^-TnTx⁺ neuronal progenitors isolated by FACS for microarray analysis in this study are represented by Jones^-Tuj1⁺ cells (population b) located near the pial surface as shown in panels 3C and 3D.