Figure 2
From: Greatly attenuated experimental autoimmune encephalomyelitis in aquaporin-4 knockout mice
Spinal cord following EAE induction. A. (left) Representative hematoxylin and eosin stained spinal cord sections (at L1 level) from EAE-induced wildtype (+/+) and AQP4 null (-/-) mice, shown at low and high (in boxes) magnifications. Arrows denote areas of mononuclear cell infiltration. All scale bars: 200 μm. (right) Histological score (see Methods) (S.E., p < 0.001). B. CD45 immunohistochemistry. Arrows denote CD45-positive lymphocytes in infiltrates. C. Luxol fast blue staining. Arrows denote regions of myelin loss. Left inset shows high magnification of Luxol fast blue staining. Right inset shows loss of NF200 immunofluorescence (red) within infiltrates (nuclei stained blue with DAPI).