Figure 5

Morphology of GABA⁺- and vGlut2⁺-neurons in neural aggregate or MNP-cultures. Examples of the morphology of non-GABAergic (white arrow heads) and GABAergic (yellow arrow heads) neurons (a) and the sub-cellular localization of vGlut2 (b) in MNP- (a, ii; b, i-iii) and neural aggregate-derived (a, i; b, iv-vi) βtubulin⁺neurons (green arrow heads) at 28 days after initiating the differentiation. Quantification of GABA⁺/βtubulin⁺ cells 7 and 28 days after initiating the differentiation (a, iii) Note, the intensive βtubulin⁺ cell labeling in neural aggregate cultures stems from the dense neurite network. Scale bar = 20 μm (a) and 10 μm (b).