Figure 4

Neuronal and glial differentiation of neural aggregate and MNP cultures. Images illustrate the composition of MNP or neural aggregate (nSFEB or SNP) cultures (a-c). Both cultures comprise nestin^-/GFAP⁺, nestin⁺/GFAP^- and nestin⁺/GFAP⁺ (a, i; b, i; c i-iii), βtubulin⁺ (a, ii; b, ii; c, iv-vi) and MBP⁺ cells (b, iii; c, iii; d, vii). Optical slices (1 μm) illustrate the morphology and localisation of nestin⁺, βtubulin⁺, GFAP⁺, NeuN⁺ and MBP⁺ cells within the core (yellow boxes) or edge (white boxes) region of secondary neural aggregates (c, i-viii). Regions of interest indicated by boxes are illustrated at higher magnifications (c, i-vi). Note, for visualisation of MBP⁺ multi-branched oligodendrocytes a set of optical slices were stacked (c, vii). Low-magnification image of nestin⁺ cells in neural aggregate cultures containing secondary neural aggregates and the interjacent cell population (c, ix) Scale bar: 75 μm. Diagrams illustrate percentages of NeuN⁺ neurons (d, i), GFAP⁺ astrocytes (d, ii) or MBP⁺ oligodendrocytes (d, iii) within neural aggregate or MNP cultures at the indicated days in vitro (div).