Table 2 Relative mRNA levels of catechol-O-methyltransferase (COMT), monoamino oxidase A (MAOA) and dopamine transporter (DAT) in SK-NF-I cells
| Â | 0 hr | 3 hrs | 6 hrs | 24 hrs | 48 hrs |
|---|---|---|---|---|---|
COMT | 1.00 ± 0.18 | 0.77 ± 0.12 | 0.95 ± 0.17 | 1.46 ± 0.23* | 0.69 ± 0.08 |
MAOA | 1.00 ± 0.11 | 1.06 ± 0.13 | 1.18 ± 0.33 | 1.88 ± 0.16** | 0.86 ± 0.10 |
DAT | 1.00 ± 0.24 | 0.78 ± 0.11 | 1.14 ± 0.15 | 1.46 ± 0.24 | 0.66 ± 0.08 |
- Parallel cell cultures were incubated in 1% hypoxia for the indicated time periods. cDNA samples were prepared from three independent cultures and amplified in three parallel PCR reactions (N = 9). Relative expression levels were calculated as means and standard deviations of
values normalized to expression levels of the RPLP0 internal control gene. Data presented in the table were generated by dividing these normalized expression levels by that of the 0-hr control (*: p < 0.05; **: p < 0.01 and ***: p < 0.001).
values normalized to expression levels of the RPLP0 internal control gene. Data presented in the table were generated by dividing these normalized expression levels by that of the 0-hr control (*: p < 0.05; **: p < 0.01 and ***: p < 0.001).