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Table 1 Selection of the optimal internal control gene for qRT-PCR assays in SK-NF-I cells.

From: Hypoxia-induced transcription of dopamine D3 and D4 receptors in human neuroblastoma and astrocytoma cells

 

RPLP0

β-actin

RPII

HMBS

HGPRT

1.04

2.83

1.17

2.49

HMBS

2.60

1.14

2.13

 

RPII

1.22

2.42

  

β-actin

2.97

   
  1. Data indicate the ratios of 2-ΔCT values of corresponding gene pairs. The ratios were calculated by dividing the greater value with the smaller one so as to get quotients exceeding 1. CT values were determined by calculating the means of three parallel PCR amplifications from three independent cDNA samples (N = 9) prepared from SK-NF-I cultures kept for 8 hrs in 1% hypoxia.
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BMC Neuroscience

ISSN: 1471-2202

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