Figure 3

Enhancement of HIF-1α activity in SK-NF-I cells upon hypoxia or DFO treatment. SK-NF-I cells were transiently transfected with the pSV40-5×HRE-luc reporter construct (Fig. 2) and incubated under normoxic or hypoxic conditions, or treated with 100 μM DFO, respectively, for 24 hours. Luciferase activities normalized to β-galactosidase levels are displayed as fold increments over the control activity. Data are means of three parallel determinations from three independent experiments (N = 9); bars represent standard deviations (*: p < 0.05; **: p < 0.01 and ***: p < 0.001).