Figure 1
Expression of HIF-1α in SK-NF-I and CCF-STTG1 cells. Parallel cultures were incubated in normoxia (21% O2, lane "N") or in hypoxia (1% O2, lane "H") for 8 hours, or were treated with 100 μM desferrioxamine for 8 hours in normoxic atmosphere (lane "DFO") where indicated. Cells were subsequently harvested, extracted and samples were resolved by SDS-PAGE, blotted and probed with an anti-HIF-1α antibody at a dilution of 1:1000. Three blots were made from 3 independent biological samples and a representative image is shown.