Immunohistochemical evaluation of midbrain damage. Experiment1: Immunohistochemistry performed using anti-tyrosine antibodies on the brain sections of animals. Representative photomicrographs of midbrain sections showing TH-immunopositive neurons from saline injected animals (A), paraquat injected water fed animals (B), paraquat injected placebo fed animals (C) and paraquat injected WS-CoQ10 fed animals (D). Magnification: 10H. Insets (a' to d') show the density of TH-immunopositive neurons and fibres in the respective groups. Magnification: 20×. (E) The number of TH-immunopositive neurons counted in every fifth section throughout the substantia nigra pars compacta. Data are shown as mean ± SEM. Note: Significant differences (p < .001) ***between saline injected-water fed group and and paraquat injected water fed group; ### between saline injected-water feds and paraquat injected-placebo fed group; HHH between paraquat injected WS-CoQ10 fed group and paraquat injected placebo fed group (evidence of neuroprotection).