Figure 7
From: The formation of acetylcholine receptor clusters visualized with quantum dots
The comparison between AChR and GM1 movement. GM1 was labeled with biotinylated cholera toxin plus streptavidin-conjugated QD (BCQ). (a) Trajectories of BBQ and BCQ over an 80-sec recording period. Note the scale of the BCQ plot was 2.5 times that of the BBQ plot. The diffusion coefficient of BCQ was nearly an order of magnitude higher that that of BBQ. (b-e) Mobile and immobile AChRs (b and d) and GM1 (c and e). Two frames in a time-lapse sequence which were separated by an interval of 2 sec were pseudocolored in red and green and superimposed. Mobile QDs appear as paired red and green dots (circles) and immobile ones as single yellow dots (arrows). QDs that blinked during this interval appeared as isolated dots with single color (arrowheads). In control cultures (b and c), diffuse AChRs and GM1 were mobile. ConA treatment resulted in immobilization of AChRs but not GM1 (d and e).