Figure 3

Immunostaining of DRG and measurement of Pt-GG adduct level. Visualization and measurement of Pt-(GG) intrastrand cross-links in the nuclear DNA of DRG neurons of C57 Bl/6 mice after chronic treatment with cisplatin (cumulative dose: 16 mg/kg). Cryosections of dorsal root ganglia were immunostained with monoclonal antibody R-C18 for Pt-(GG) adducts (B) and with DAPI for nuclear DNA (A). Arrows indicate nuclei of neurons. C: Measurement of Pt-(GG) adduct levels in DRG neurons of mice treated with Cisplatin alone or in combination with rhEPO by quantitative image analysis of nuclear signals (AFU: arbitrary fluorescence units normalised for the DNA content of individual cells); means of >200 nuclei +/- SD.