Figure 3

NET immunoreactivity colocalizes with VMAT2 in the hippocampus. Images were collected from the dentate gyrus of the mouse hippocampus. Sections were immunostained using NET-05 (A, green) and VMAT2 antibody (B, red). Panel C is a merge of A and B. Extensive colocalization of VMAT2 and NET is evident on many fibers. Essentially all VMAT2 positive puncta colocalize with NET immunoreactivity. In contrast, some NET-positive fiber segments appear to have low or absent VMAT2 staining (note green profiles in C inset). Insets shows a digital zoom of each field. Scalebar A-C = 50 μM. Scalebar for inset panels = 10 μM.