Figure 2

NET colocalizes with TH in the locus ceruleus and bed nucleus of the stria terminalis. Mouse brain sections were processed for confocal-assisted imaging of NET immunoreactivity as described in Methods. A-C. Coronal sections encompassing the locus ceruleus (LC) were doubled-labeled using antibodies against both tyrosine hydroxylase (TH, red) (A, D) and NET (B, E, green). Panels C and F are merged images of A/B and D/E, respectively. Arrow in B identifies a dual labeled LC dendrite. Scalebar in A-C = 200 μm; scalebar in D-F = 20 μm. G-I. Projections of six confocal sections (63×) taken from the dorsal BNST (dBNST). TH and NET immunoreactivities are shown in panel G (red) and panel H (green), respectively. Panel I is the merge of G and H. Insets of G-I show a digital zoom to illustrate individual fibers. J-L: Projections of six confocal sections (63×) taken from the ventral BNST (vBNST). TH immunoreactivity is shown in panel J (red) and NET immunoreactivity is shown in panel K and the overlap in panel L. All scalebar = 20 μM.