Morphometric analysis of NGF effect on optic nerve diameter (A), ganglion cells (B), betaIII-Tubulin (C) and MBP (D) immunostaining. NGF administration partially prevents 2VO-induced optic nerve atrophy (A) and ganglion cells degeneration at 75 days after surgery. NGF also induces a partially recovery of MBP- immunoreactivity (D), whereas betaIII-tubuline immunostaining is not modified (C). Statistical analysis: one-way ANOVA and post hoc Tukey's test: #p < 0.05; ##p < 0.01 and Student-t test: *p < 0.05.).