Table 3 Details of qPCR and genotyping assay primers and conditions
From: Genes within the serotonergic system are differentially expressed in human brain
Gene | Assay on demand ID | Genotype variant | Genotype detection method | Genotyping primers (forward and reverse) | Size (bp) | Temp °C | MgCl2 (mM) |
|---|---|---|---|---|---|---|---|
TPH1 | Hs00188220_m1 | T-346G | MslI enzyme digest | 5'CTTCGTTATGTGTACAGTCC-3' 5'TAGGACTGCAGTGCTTCTC-3' | G = 365 T = 305+60 | 59 | 2 |
TPH2 | Hs00542783_m1 | G-703T | Taqman allelic discrimination | 5'ACACTCACACATTTGCATGCAC-3' 5'CATTGACCAACTCCATTTTATGTTAATAAGCT-3' | |||
TPH2 taqman genotyping probe sequences: VIC-CTTGACATATTCTAATTTT FAM-ACTTGACATATTATAATTTT | |||||||
5-HT2A | Hs00167241_m1 | A-1438G | MspI enzyme digest | 5'AAGCTGCAAGGTAGCAACAGC-3' 5'AACCAACTTATTTCCTACCAC-3' | A = 468 G = 244+224 | 56 | 1.5 |
5-HT2C | Hs00168365_m1 | GTn promoter microsatellite -1027 bp | capillary electrophoresis | FAM5'GGGAGTTTCAAAGCTTGATGA3' 5'GTTTCTTAGACCCATGGTGGAGATGG-3' | ~259; Z-6 allele | 59.5 | 2.5 |
5-HTT | Hs00169010_m1 | 5-HTTLPR | agarose gel electrophoresis | 5'ATGCCAGCACCTAACCCCTAATGT-3' 5'GGACCGCAAGGTGGGCGGGA-3' | 419; 16 rpt 'L' | 66 | 1.5 |
intron2 VNTR | agarose gel electrophoresis | 5'GTCAGTATCACAGGCTGCGAG-3' 5'TGTTCCTAGTCTTACGCCAGT-3' | STin12 ~299 | 54 | 1.5 | ||
GAPDH | Hs00266705_g1 | n/a | |||||
UBC | Hs00824723_m1 | n/a | |||||
SDHA | Hs00188166_m1 | n/a |