Figure 2

RXR ligand rescues primary DA neurons from hypoxia, but not from KA or H ₂ O ₂ -induced toxicity. A) Hypoxia induced a time dependent decrease in the number of primary DA cells, giving 18%, 42% and 49% reduction after 7, 24 and 36 hours in 0-1% O₂, respectively. B) LG268 treatment (grey bars) during hypoxic conditions partly rescued the DA cells, giving a 30% and 40% increase in TH⁺ cells compared to the cultures without added ligand after 24 and 36 hours, respectively. C) KA exposure at 100 and 500 μM reduced the number of primary DA cells by 52% and 98% compared to untreated cultures, respectively (black bars). LG268 provided no rescue (grey bars). D) H₂O₂ exposure at 80 and 110 μM gave 70 and 83% neurodegeneration, respectively (black bars). This could not be restored by addition of LG268 (grey bars). Stars (*) refer to differences between control-group (N2) and experimental-group, whereas values above brackets refer to differences between different experimental-groups. ns denotes non-significant, * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 according to Tukey's multiple comparison test.