Table 1 List of primary and secondary antibodies used for immunocytochemistry
From: Progranulin is expressed within motor neurons and promotes neuronal cell survival
ANTIBODY | TITRE | SOURCE | SECONDARY |
|---|---|---|---|
P GRN | 1:500 | R&D SYSTEMS | DONKEY-ANTI-SHEEP (ALEXA FLUOR 488 or 594 |
T DP -43 | 1:100 | PROTEINTECH | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488) |
C YTOCHROME C | 1:400 | PHARMINGEN | DONKEY-ANTI-MOUSE (ALEXA FLUOR 488 OR 594) |
C ALRETICULIN | 1:400 | STRESSGEN | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488 OR 594) |
G M 130 | 1:300 | PHARMINGEN | DONKEY-ANTI-MOUSE (ALEXA FLUOR 488 OR 594) |
C HROMOGRANIN A | 1:100 | RDI | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488) |
S NAP -25 | 1:1000 | SIGMA | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488) |
S YNAPTOPHYSIN | 1:200 | SIGMA | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488) |
C D 11b | 1:100 | BIO LEGENDS | NONE -(PRIMARY LABELED WITH ALEXA-FLUOR 488) |
G FAP | 1:400 | PROTEINTECH | DONKEY-ANTI-RABBIT (ALEXA FLUOR 488) |
S MI 32 | 1:1000 | STERNBERGER | DONKEY-ANTI-MOUSE (ALEXA FLUOR 488) |