Figure 3

Protein levels of NADPH-cytochrome P450 reductase and for cellular oxidoreductases in CTX and CbG neurons. (A) Protein levels of NADPH-cytochrome P450 reductase in neurons. Immune labeling with anti-cytochrome P450 reductase antibodies was performed on proteins extracted from neurons cultured under conditions of 20% O₂ tension. Results of densitometric measurements of the immuno-reactive band in each immunoblot are shown as the mean (± SEM) of 5 experiments. Statistically significant differences (t-test) are shown for the indicated comparisons. (B) Protein levels of SOD1, SOD2, and GPX1 in neurons. Immune labeling with anti-SOD1, anti-SOD2, and anti-GPX1 antibodies was performed on proteins extracted from neurons cultured as described in A. Results of densitometric measurements of the immuno-reactive bands in each immunoblot are shown as the mean (± SEM) of 3 experiments. Statistically significant differences (t-test) are shown for the indicated comparisons. (C) Effect of paraquat-induced OS on the protein levels of SOD1, SOD2, and GPX1 in neurons. Primary neurons in culture were exposed to the indicated concentrations of paraquat for 24 h at 37°C. Immune labeling with antibodies was performed as described in B. Results of densitometric measurements of the immuno-reactive bands in each immunoblot are shown as the mean (± SEM) of 3–5 experiments. Statistically significant differences (t-test) are shown for the indicated comparisons.