Figure 3
From: Labelling and targeted ablation of specific bipolar cell types in the zebrafish retina
Enhancer trap lines express eGFP in different types of bipolar cells in larvae. Retinal cryosection of Tg(Gal4-VP16;UAS:eGFP)xfz 3larva at 5 dpf (A-C). (A) Confocal image of cross-section (dorsal at the top) stained with an anti-GFP antibody (green) and counterstained with DAPI (blue). (B) Higher magnification image of the central part of the retina shown in (A). (C) Same confocal image as in (B) without DAPI. (D-F) Retinal cryosection from a Tg(Gal4-VP16;UAS:eGFP)xfz 43larva at 5 dpf. The confocal images are arranged in the same way as shown for the other line. Scale bar: 50 μm (A, D); 25 μm (B, C, E, F). Abbreviations: gcl, ganglion cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; L, lens; onl, outer nuclear layer.