Fig. 2

Protein expression at choroid plexus. Western blots on rat CP from males, MET and ES females (A), where membranes are cut by molecular weight and the resultant images cropped to show representative bands. ATP1a1, lamin and AQP1 columns represent 1 set of gels ran in parallel and the same well number on each membrane. Lamin and CAII represent another set of gels ran in parallel and the same well number on a membrane. Expression of total AQP1 (B), non-glycosylated AQP1 (C), glycosylated AQP1 (D), non-glycosylated/glycosylated ratio (E), carbonic anhydrase 2 (CAII) (F) and ATP1a1 (G). One-way ANOVA with post-hoc Holm-Sidak’s test for B, C and D. Kruskal–Wallis test with post-hoc Dunn’s test for E, F and G. Data presented as mean ± SD. N = 8–10. *p < 0.05, **p < 0.01 and ***p < 0.001