Differentiation of human bone marrow stem cells into cells with a neural phenotype: diverse effects of two specific treatments
© Scintu et al; licensee BioMed Central Ltd. 2006
Received: 12 October 2005
Accepted: 16 February 2006
Published: 16 February 2006
It has recently been demonstrated that the fate of adult cells is not restricted to their tissues of origin. In particular, it has been shown that bone marrow stem cells can give rise to cells of different tissues, including neural cells, hepatocytes and myocytes, expanding their differentiation potential.
In order to identify factors able to lead differentiation of stem cells towards cells of neural lineage, we isolated stromal cells from human adult bone marrow (BMSC). Cells were treated with: (1) TPA, forskolin, IBMX, FGF-1 or (2) retinoic acid and 2-mercaptoethanol (BME). Treatment (1) induced differentiation into neuron-like cells within 24 hours, while a longer treatment was required when using retinoic acid and BME. Morphological modifications were more dramatic after treatment (1) compared with treatment (2). In BMSC both treatments induced the expression of neural markers such as NF, GFAP, TUJ-1 and neuron-specific enolase. Moreover, the transcription factor Hes1 increased after both treatments.
Our study may contribute towards the identification of mechanisms involved in the differentiation of stem cells towards cells of neural lineage.
Up until only a few years ago, the fate of adult stem cells was believed to be limited to their tissue of origin. However, it has recently been demonstrated that in several tissues adult stem cells may undergo a fate other than that habitually manifested in physiological conditions. As an example, stem cells isolated from bone marrow can differentiate not only into blood cells, but also into hepatocytes , skeletal muscle  and cardiomyocytes . Even more surprisingly, recent studies have shown that stem cells in vivo may even give rise to neural cells, thus representing an important alternative to embryonic cells for therapeutic transplantation [4, 5]. Indeed, the original idea of substituting neurons damaged by different pathological events with undamaged heterologous neurons has not so far afforded satisfactory results [6, 7]. On the other hand, although the discovery of the existence of neural stem cells in the adult human brain [for a review see ] is certainly a milestone in the field, it is difficult to conceive a method leading to manipulation and reimplantation of neural stem cells taken directly from the patient's brain. Accordingly, the source represented by bone marrow offers theoretically unlimited therapeutical applications.
Two major subpopulations of stem cells can be identified in bone marrow: hematopoietic stem cells, which give rise to red blood cells, platelets, monocytes, granulocytes and lymphocytes, and non-hematopoietic stromal cells (BMSC), more recently acknowledged to be of a mesenchymal nature due to their capacity to differentiate into myogenic, osteogenic, chondrogenic and adipogenic lineages [9, 10]. BMSC can be isolated from whole bone marrow by adherence to plastic culture dishes. Recent studies have shown that BMSC can be induced to express a neuronal phenotype in vitro under specific experimental conditions. For example, Woodbury et al.  observed that in the presence of β-mercaptoethanol and dimethylsulfoxide BMSC may differentiate into cells expressing neuron specific enolase (NSE) and neurofilament. Moreover, it has been described that in the presence of epidermal growth factor (EGF) and brain-derived neurotrophic factor (BDNF) stromal cells differentiate into neural cells that express both neuronal and glial markers (Neu-N, nestin, GFAP) . Deng et al.  demonstrated how factors that increase intracellular cAMP induce the differentiation of BMSC into early progenitors of neural cells. Unfortunately, due to the differences in BMSC isolation and culture conditions, as well as in characterization of differentiated cells, it is not possible to compare these treatments. For this reason, we examined the different effects produced by two specific treatments in inducing differentiation of BMSC into neural phenotype. Recent studies show that the transcription factors with helix-loop helix (bHLH) motifs [for review see: [14, 15]] are essential for maintenance of neural precursor cells in an undifferentiated state. bHLH genes include the mammalian Hairy and Enhancer of Split genes (Hes1, Hes3 and Hes5); in particular Hes1 is highly expressed by neural stem cells [15, 16]. Our results show that Hes1 is involved in BMSC differentiation into neural cells. In fact a transient increase of this transcription factor was observed in neural precursors after both treatments.
Induction of neural phenotype
RT-PCR for Hes1 was performed in untreated and treated BMSC. The graph in fig. 4 shows that Hes1 was expressed at low levels in undifferentiated BMSC, but its mRNA levels significantly increased after 5 hours of treatment1. Hes1 expression tended to decrease as neural differentiation proceeds. In fact, after 24 h and 48 h of treatment 1, mRNA levels were not significantly different as compared to undifferentiated cells. At the same manner, treatment 2 determined a significant increase of Hes1 expression after 7 days.
In this study we induced stromal cells from human bone marrow to differentiate into cells with a neural phenotype, comparing two different protocols. Furthermore, we explored for the first time the involvement of the transcription factor Hes1 in inducing neural phenotype in BMSC. Both treatments led to a neural phenotype in a similar but not identical manner. Indeed, at the end of both treatments, BMSC expressed neural markers such as NF, TUJ-1 and GFAP, whereas nestin and vimentin progressively decreased with differentiation. These results were associated with different morphological modifications and required times of exposure ranging from one day to one week, depending on the treatment. The first induction media (a cocktail of PKC and PKA activators and FGF-1) rapidly determined dramatic modifications of cell shape and induced the expression of several neural markers in less than 24 hours. Differentiation was transient and cells reverted to their original phenotype when the inducing factors were removed. On treating BMSC with retinoic acid and BME biochemical markers consistent with differentiation towards a neural phenotype were not expressed before seven days. Moreover, morphological changes were not as drastic as in the other treatment, although differentiation persisted even when inducing agents were removed.
It is well known that an increase of intracellular cAMP and consequent PKA activation represents a critical regulator for differentiation of neurons and glia . Moreover, agents that increase intracellular cAMP levels induce neuroendocrine differentiation in human prostate carcinoma cells , neuronal differentiation in C6 glioma cells [19, 20] and processes elongation in MCD-1 medulloblastoma cell line . In the same manner, PKC is involved in neural differentiation regulating neurite outgrowth and branching [22, 23]. Iacovitti et al.  showed that FGF-1, together with co-activator molecules such as dopamine, PKA and PKC activators, induced differentiation of an embryonic carcinomal stem cell line into dopamine neurons. Deng et al.  found that human BMSC can be differentiated into early neural progenitors by conditions that increase intracellular cAMP. After six days of treatment these cells expressed NSE and vimentin but not markers of mature neurons, such as NF-M or MAP-2. In our experiments, treatment 1 induced BMSC to differentiate into more mature neuron-like cells within 24 hours.
Retinoic acid (RA), a derivative of vitamin A, is essential in maintaining normal cellular growth and development. In fact, RA is present in various tissues of both embryonic and adult animals, in particular in the nervous system [25–30], where it promotes neuronal differentiation . Previous studies have demonstrated that RA induces both a greater number of neurites as well as increased neurite length in cultured neurons [for a review, see ]. Retinoic acid has been used in combination with other factors to induce differentiation of BMSC into neural cells [12, 33–35]. Since it has been suggested that BME is capable of supporting the viability and differentiation of fetal mouse brain neurons , we used low concentrations of this factor in combination with retinoic acid (treatment 2). With this treatment, BMSC slowly differentiated into neuron-like cells and after 7 days they expressed NF-M. Cells survived in induction media for up to at least 14 days.
These results confirmed data reported by other authors suggesting that BMSC are able to differentiate in cell types of varying embryonic origin. In particular, using two different treatments we induced BMSC to differentiate into cells with neuronal phenotype. At the end of both treatments cells expressing neural markers such as neurofilament and GFAP were obtained, whereas vimentin and nestin decreased with differentiation. The presence of GFAP in treated BMSC may be ascribed not only to differentiation into astrocytes, but also to neural precursors, that have been demonstrated to express this protein .
Prior to inducing differentiation, BMSC expressed nestin, a commonly used marker of neural precursor. A transient expression of this intermediate filament in non-neural cells, such as hepatic stellate cells, myogenic and epithelial cells has recently been observed [38, 39]. Moreover, in our experiments the patterns of mRNA and protein expression did not exactly match. For example, mRNA for neurofilaments was present in BMSC and was not modified during differentiation, whereas the protein was absent in untreated cells and its expression was induced by treatments. These data suggest that the expression of neural markers (such as neurofilaments, nestin, GFAP, NSE) is controlled at a translational rather than a transcriptional level. In fact, the constitutive expression of these proteins by BMSC confirms the hypothesis that these cells are "multidifferentiated" cells and thus can retain the ability for neuronal differentiation, as already suggested by Tondreau et al. .
Microscopic examination of the cultures revealed that, using either of the two protocols, only a small percentage of cells (15–20%) showed immunoreactivity for neural markers, suggesting that only a subset of BMSC can differentiate into cells with a neural phenotype. We can therefore hypothesize that this subfraction of BMSC may be constituted by tissue-specific progenitor cells with restricted differentiation potential, capable of giving rise, under specific experimental conditions, to cells characteristically found in other tissues. In fact, morphological and phenotypic heterogeneity of BMSC has been demonstrated: BMSC include small round cells and large polygonal-shaped cells with different multilineage potentials [41, 42]. On the other hand, our findings may indicate the possibility that a population of multipotential stem cells resides in adult bone marrow.
The results reported demonstrate how the microenvironment may be capable of affecting cellular differentiation, since activation of PKs alone leads to neuron-like cell morphology, but the cells express neural markers after both treatment protocols. This observation suggests that specific marker expression is not always accompanied by typical morphological modifications [5, 43]. In contrast, Black and Woodbury  have demonstrated that BME induces stromal rat cells to differentiate into neuronal phenotype cells, but at concentrations 10–100 times higher than those used in the present study. In our experiments, RA and BME concentrations were sufficient to affect the expression of neural markers but not to induce morphological modifications. The differences observed between the two treatments respect to morphological changes and to time required for differentiation may indicate the activation of different intracellular molecular mechanisms in these processes. It has been suggested that the molecular mechanisms involved in the action of RA during embryogenesis may occur via a complex signaling pathway [for review see ]; moreover, it has been hypothesized that both in vitro and in vivo, the number of neural-specific genes encoding transcription factors and signaling molecules induced by RA during neural differentiation are comparable .
In a recent report Lu et al.  observed a neuron-like morphology in BMSC exposed to various stressors. An increase in NSE immunoreactivity was also observed, but it could not be confirmed by RT-PCR, suggesting that these modifications can be the result of cell shrinkage. On the contrary, our results showed the increase of neural markers not only by immunocytochemistry, but also by RT-PCR and western blot.
Hes1 is a transcription factor that plays a key role in neurogenesis regulation maintaining cortical progenitors by inhibiting neurogenesis . Our results show that after 5 hours of treatment 1, the expression of Hes1 transiently increases. We could speculate that the upregulation of Hes1 expression induces differentiation of BMSC into neural precursors and its inhibition leads these cells to differentiate into more mature cells, as observed in vivo during development . On the other hand, we observed an increase of Hes1 expression after 7 days of treatment 2. This result may indicate that retinoic acid treatment leads cells to acquire a less mature phenotype as compared to treatment 1. This hypothesis is confirmed by nestin expression that decreases after treatment 1, but that is not modified by treatment 2.
As summarized in the present report, it has been demonstrated that different differentiation-inducing factors and protocols are capable of generating neural cell types from BMSC. Moreover, we observed a modulation of Hes1 expression with both treatment, suggesting an involvement of this transcription factor in differentiation of BMSC into neural cells. By combining these molecular biological strategies and neuronal differentiation systems, it should prove possible to detect, isolate, and characterize specifically expressed genes involved in neuronal differentiation. Our results suggest that adult bone marrow stem cells may differentiate into cells with distinct glial or neuronal phenotypes after exposure to specific signals or modulating specific genes, such as Hes1, and thus constitute a cellular reservoir for neurodegenerative diseases of the central nervous system.
20–40 ml bone marrow aspirates were taken from 10 healthy donors (age range 6–33 years) after obtaining of informed consent. Mononuclear cells were separated by gradient density using Ficoll-Hypaque and MSC selected by plastic adhesion according to Lee et al. . Briefly, cells were seeded in culture flasks including α-MEM (Cambrex) supplemented with 20% FCS and 10-8M vitamin K. After 24 hours, 10-8M dexamethasone and 50 μg/ml ascorbic acid were added without media change. Subsequently, the culture flasks were left undisturbed for 4–5 days to promote cell attachment. After this time, the non-adherent cells were removed by replacing the medium. At near-confluence, cells were subcultured after trypsin digestion.
After at least four passages, cells were plated at a density of 2 × 104 cells/ml in a 10-cm plastic culture dish for western blot and RT-PCR, and in 3.5-cm dishes containing glass coverslips for immunocytochemistry and electron microscopy. After 24 hours, cells were treated with two different protocols:
10 ng/ml FGF-1 (R&D, Minneapolis, MN), 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA; Sigma, St Louis, USA), 250 μM IBMX (Sigma) and 50 μM forskolin (Sigma), in DMEM/F12 (Sigma) 1:1 supplemented with ITS (Sigma) 1%.
1 μM all-trans-retinoic acid (Sigma) and 100 μM 2-mercaptoethanol in DMEM supplemented with 10% FCS (Invitrogen).
At various time intervals, cultures were fixed for immunocytochemistry and electron microscopy, or processed for RT-PCR and western blot.
Cells on coverslips were fixed at -20°C with cold methanol for 4 min. For staining, samples were rehydrated in PBS/0.2% (v/v) Triton X-100 and pre-incubated with normal goat serum (1:5, Vector, Burlingame, CA, USA) for 30 min. Cells were then overlaid with the following primary antibodies diluted in PBS/0.2% (v/v) Triton, for 60 min at room temperature: monoclonal anti-nestin (1:100, Chemicon, Temecula, CA, USA); polyclonal anti-GFAP (1:200, DAKO, Carpinteria, CA, USA); polyclonal anti-neurofilament 150 kDa (1:100, Chemicon). Texas red conjugated anti-mouse IgG (1:200; Jackson ImmunoResearch, West Grove, PA, USA) or FITC conjugated anti-rabbit IgG (1:500; Molecular Probes, Eugene, OR, USA) were used as secondary antibodies. After several washings samples were overlaid with Hoechst 33258. Negative controls were incubated with non immune serum and with appropriate secondary antibody.
In other experiments, cells were incubated with 1% phenylhydrazine hydrochloride (Sigma) to block endogenous peroxidase for 30 min and then with monoclonal antibodies against neuron specific enolase (NSE; 1:20, Cymbus Biotechnology) for 60 min at room temperature. Subsequently, slides were rinsed three times in PBS-Triton, incubated for 30 min with biotinylated antimouse IgG (1:200, Vector), rinsed and incubated with avidin D-conjugated horseradish peroxidase (1:800, Vector) followed by 10 min incubation with DAB tablet sets (SIGMA FAST). Negative controls were incubated with non immune serum instead of primary antibody.
Cells were lysed with sodium dodecyl sulphate (SDS) 2%. Protein concentration was measured according to the method of Lowry . Loading buffer (75 mM Tris-HCl, pH 6.8, 20 % glycerol, 5% 2-mercaptoethanol, 0.001 bromophenol blue) was subsequently added and samples were placed in boiling water for 3 min. 15–30 μg of protein were run on 10% or 5% SDS-polyacrilamide gel, depending on molecular weight. Separated proteins were electroblotted onto PVDF membrane (Hybond-P, Amersham) and blocked with 5% nonfat dry milk overnight at 4°C. Immunodetection was performed with antibodies against nestin (1:1000, monoclonal, Chemicon, Temecula, CA, USA), GFAP (1:5000, polyclonal, DAKO, Carpinteria, CA, USA), neurofilament (1:5000, polyclonal, Chemicon), vimentin (1:1000, monoclonal, DAKO, Carpinteria, CA, USA), NSE (1:5000, monoclonal, Cymbus Biotechnology), TUJ-1 (β-III tubulin; 1:500, monoclonal, Chemicon) and GAPDH (1:600, monoclonal, Chemicon). The membrane was washed and incubated with a horseradish peroxidase conjugated anti-mouse IgG (1:1000, Chemicon) or anti-rabbit IgG (1:5000, Chemicon). After washing, protein bands were detected with SuperSignal West Pico chemiluminescent substrate (Pierce).
Reverse transcription-polymerase chain reaction (RT-PCR)
Primer sequences and cycling conditions in RT-PCR
Lenght of amplified
30 sec, 94°C
30 sec, 55°C
30 sec, 72°C
30 sec, 94°C
30 sec, 55°C
30 sec, 72°C
30 sec, 94°C
60 sec, 55°C
90 sec, 72°C
60 sec, 94°C
60 sec, 60°C
120 sec, 72°C
30 sec, 94 °C
30 sec, 60 °C
30 sec, 72 °C
30 sec, 96°C
60 sec, 58°C
30 sec, 74°C
Fluorescence-activated cell sorting (FACS)
For surface protein expression, BMSC were detached with 0.5% trypsin for 10–15 min. at 37°C, washed, counted and resuspended in 0.1% BSA/PBS and incubated with the following primary antibodies: monoclonal phycoerythrin-conjugated anti-CD34 (Milteny Biotec); monoclonal phycoerythrin-conjugated anti-CD13; monoclonal anti-CD90 (BD Biosciences Pharmigen), CD73 (BD Biosciences Pharmigen), CD105 (DAKO) and CD11b (DAKO). Non-conjugated mAbs were stained after washing in 0.1% BSA/PBS with FITC-conjugated anti-mouse IgG (1:1000, Chemicon). After washing, cell fluorescence signals were determined immediately using a FACScan flow cytometer (Becton Dickinson, San Jose, CA, USA) equipped with an argon laser emission of 488 nm. The analysis was performed using CellQuest Software (Becton Dickinson, San Jose, CA, USA).
Scanning Electron Microscopy (SEM)
Cultured cells were prefixed in a solution containing 1% paraformaldeyde, 1.5% glutaraldehyde in 0.15 M cacodylate buffer for 2 hours at room temperature. After washes with PBS, samples were dehydrated with acetone and critical point dried using carbondioxide. Specimens were then briefly coated with platinum in a Emitek K575-E sputtering apparatus and observed with a Hitachi S4000 FE scanning electron microscope.
This work was supported by MIUR (FIRB and FISR) and Regione Autonoma Sardegna grant
We thank Dr. Felice Loffredo for his technical help in scanning electron microscopy and Dr. Roberto Serpe for FACS analysis
- Petersen BE, Bowen WC, Patrene KD, Mars WM, Sullivan AK, Murase N, Boggs SS, Greenberger JS, Goff JP: Bone marrow as a potential source of hepatic oval cells. Science. 1999, 284: 1168-1170. 10.1126/science.284.5417.1168.View ArticlePubMed
- Ferrari G, Cusella-DeAngelis G, Coletta M, Paolucci E, Stornaiuolo A, Cossu G, Mavilio F: Muscle regeneration by marrow-derived myogenic precursors. Science. 1998, 279: 1528-1530. 10.1126/science.279.5356.1528.View ArticlePubMed
- Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Nickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P: Bone marrow cells regenerate infarcted myocardium. Nature. 2001, 410: 701-705. 10.1038/35070587.View ArticlePubMed
- Mezey E, Chandross KJ, Harta G, Maki RA, McKercher SR: Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science. 2000, 290: 1779-1782. 10.1126/science.290.5497.1779.View ArticlePubMed
- Brazelton TR, Rossi FM, Keshet GI, Blau HM: From marrow to brain: expression of neuronal phenotypes in adult mice. Science. 2000, 290: 1775-1779. 10.1126/science.290.5497.1775.View ArticlePubMed
- Defer GL, Geny C, Ricolfi F, Fenelon G, Monfort JC, Remy P, Villafane G, Jeny R, Samson Y, Keravel Y, Gaston A, Degos JD, Peschanski M, Cesaro P, Nguyen JP: Long-term outcome of unilaterally transplanted parkinsonian patients. I. Clinical approach. Brain. 1996, 119: 41-50.View ArticlePubMed
- Lopez-Lozano JJ, Bravo G, Brera B, J Dargallo, J Salmean, J Uria, J Insausti, R Martinez, P Sanchez, de la Torre C, R Moreno: Regression of parkinsonian fetal ventral mesencephalon grafts upon withdrawal of cyclosporine A immunosuppression. the CPH Neural Transplantation Group Transplant Proc. 1999, 29: 977-80.
- Gage FH: Mammalian neural stem cells. Science. 2000, 287: 1433-1438. 10.1126/science.287.5457.1433.View ArticlePubMed
- Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 1999, 284: 143-147. 10.1126/science.284.5411.143.View ArticlePubMed
- Colter DC, Class R, DiGirolamo CM, Prockop DJ: Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci U S A. 2000, 97: 3213-3218. 10.1073/pnas.070034097.PubMed CentralView ArticlePubMed
- Woodbury D, Schwarz EJ, Prockop DJ, Black IB: Adult rat human bone marrow stromal cells differentiate into neurons. J Neurosci Res. 2000, 61: 364-370. 10.1002/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C.View ArticlePubMed
- Sanchez-Ramos JR, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel NR, Cooper D, Sanberg PR: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol. 2000, 164: 247-256. 10.1006/exnr.2000.7389.View ArticlePubMed
- Deng W, Obrocka M, Fisher I, Prockop DJ: In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Biochem Biophys Res Commun. 2001, 282: 148-152. 10.1006/bbrc.2001.4570.View ArticlePubMed
- Kageyama R, Ohtsuka T, Hatakeyama J, Ohsawa R: Roles of bHLH genes in neural stem cell differentiation. Exp Cell Res. 2005, 306: 343-348. 10.1016/j.yexcr.2005.03.015.View ArticlePubMed
- Ross SE, Greenberg ME, Stiles CD: Basic helix-loop-helix factors in cortical development. Neuron. 2003, 39: 13-25. 10.1016/S0896-6273(03)00365-9.View ArticlePubMed
- Ishibashi M, Ang SL, Shiota K, Nakanishi S, Kageyama R, Guillemot F: Targeted disruption of mammalian hairy and Enhancer of split homolog-1 (HES-1) leads to up-regulation of neural helix-loop-helix factors, premature neurogenesis, and severe neural tube defects. Genes Dev. 1995, 9: 3136-3148.View ArticlePubMed
- Kim G, Choe Y, Park J, Cho S, Kim K: Activation of protein kinase A induces neuronal differentiation of HiB5 hippocampal progenitor cells. Brain Res Mol Brain Res. 2002, 109: 134-145. 10.1016/S0169-328X(02)00550-8.View ArticlePubMed
- Bang YJ, Pirnia F, Fang WG, Kang WK, Sartor O, Whitesell L, Ha MJ, Tsokos M, Sheahan MD, Nguyen P: Terminal neuroendocrine differentiation of human prostate carcinoma cells in response to increased intracellular cyclic AMP. Proc Natl Acad Sci USA. 1994, 91: 5330-5334.PubMed CentralView ArticlePubMed
- Sharma SK, Raj AB: Transient increase in intracellular concentration of adenosine 3':5'-cyclic monophosphate results in morphological and biochemical differentiation of C6 glioma cells in culture. J Neurosci Res. 1997, 17: 135-141. 10.1002/jnr.490170207.View Article
- Ghosh P, Singh UN: Intercellular communication in rapidly proliferating and differentiated C6 glioma cells in culture. Cell Biol Int. 1997, 21: 551-557. 10.1006/cbir.1997.0193.View ArticlePubMed
- Moore KD, Dillon-Carter O, Conejero C, Poltorak M, Chedid M, Tornatore C, Freed WJ: In vitro properties of a newly established medulloblastoma cell line, MCD-1. Mol Chem Neuropathol. 1996, 29: 107-126.View ArticlePubMed
- Cabell L, Audesirk G: Effects of selective inhibition of protein kinase C, cyclic AMP-dependent protein kinase, and Ca(2+)-calmodulin-dependent protein kinase on neurite development in cultured rat hippocampal neurons. Int J Dev Neurosci. 1993, 11: 358-368.View Article
- Audesirk G, Cabell L, Kern M: Modulation of neurite branching by protein phosphorylation in cultured rat hippocampal neurons. Brain Res Dev Brain Res. 1997, 102: 247-260. 10.1016/S0165-3806(97)00100-4.View ArticlePubMed
- Iacovitti L, Stull ND, Jin H: Differentiation of human dopamine neurons from an embryonic carcinomal stem cell line. Brain Res. 2001, 912: 99-104. 10.1016/S0006-8993(01)02723-8.View ArticlePubMed
- Wagner M, Han B, Jessell TM: Regional differences in retinoid release from embryonic neural tissue detected by an in vitro reporter assay. Development. 1992, 116: 55-66.PubMed
- Horton C, Maden M: Endogenous distribution of retinoids during normal development and teratogenesis in the mouse embryo. Dev Dyn. 1995, 202: 312-323.View ArticlePubMed
- McCaffery P, Drager UC: Hot spots of retinoic acid synthesis in the developing spinal cord. Proc Natl Acad Sci U S A. 1994, 91: 7194-7197.PubMed CentralView ArticlePubMed
- McCaffery P, Drager UC: Retinoic acid synthesizing enzymes in the embryonic and adult vertebrate. Adv Exp Med Biol. 1995, 372: 173-183.View ArticlePubMed
- Yamamoto M, McCaffery P, Drager UC: Influence of the choroid plexus on cerebellar development: analysis of retinoic acid synthesis. Brain Res Dev Brain Res. 1996, 93: 182-190. 10.1016/0165-3806(96)00038-7.View ArticlePubMed
- Maden M, Sonneveld E, van der Saag PT, Gale E: The distribution of endogenous retinoic acid in the chick embryo: implications for developmental mechanisms. Development. 1998, 125: 4133-4144.PubMed
- Takahashi J, Palmer TD, Gage FH: Retinoic acid and neurotrophins collaborate to regulate neurogenesis in adult-derived neural stem cell cultures. J Neurobiol. 1999, 38: 65-81. 10.1002/(SICI)1097-4695(199901)38:1<65::AID-NEU5>3.0.CO;2-Q.View ArticlePubMed
- Maden M: Role and distribution of retinoic acid during CNS development. Int Rev Cytol. 2001, 209: 1-77.View ArticlePubMed
- Locatelli F, Corti S, Donadoni C, Guglieri M, Capra F, Strazzer S, Salani S, Del Bo R, Fortunato F, Bordoni A, Comi GP: Neuronal differentiation of murine bone marrow Thy-1- and Sca-1-positive cells. J Hematother Stem Cell Res. 2003, 12: 727-734. 10.1089/15258160360732740.View ArticlePubMed
- Hung SC, Cheng H, Pan CY, Tsai MJ, Kao LS, Ma HL: In vitro differentiation of size-sieved stem cells into electrically active neural cells. Stem Cells. 2002, 20: 522-529. 10.1634/stemcells.20-6-522.View ArticlePubMed
- Kim BJ, Seo JH, Bubien JK, Oh YS: Differentiation of adult bone marrow stem cells into neuroprogenitor cells in vitro. Neuroreport. 2002, 13: 1185-1188. 10.1097/00001756-200207020-00023.View ArticlePubMed
- Ishii K, Katamaya M, Hori K, Yodoi J, Nakanishi T: Effects of 2-mercaptoethanol on survival and differentiation of fetal mouse brain neurons cultured in vitro. Neurosci lett. 1993, 163: 159-162. 10.1016/0304-3940(93)90371-Q.View ArticlePubMed
- Goldman S: Glia as neural progenitor cells. Trends Neurosci. 2003, 26: 590-596. 10.1016/j.tins.2003.09.011.View ArticlePubMed
- Niky T, Pekny M, Hollemans K, Bleser PD, Berg KV, Vaeyens F, Quartier E, Schuit F, Geerts A: ClassVI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells. Hepatology. 1999, 29: 250-257. 10.1002/hep.510290122.View Article
- Mokry J, Nemecek S: Immunohistochemical detection of intermediate filament nestin. Acta Medica. 1998, 47: 73-80.
- Tondreau T, Lagneaux L, Dejeneffe M, Massy M, Mortier C, Delforge A, Bron D: Bone marrow-derived mesenchymal stem cells already express specific neural proteins before any differentiation. Differentiation. 2004, 72: 319-326. 10.1111/j.1432-0436.2004.07207003.x.View ArticlePubMed
- Vogel W, Grunebach F, Messam CA, Kanz L, Brugger W, Buhring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003, 88: 126-133.PubMed
- DC Colter, Sekiya I, Prockop DJ: Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci U S A. 2001, 98: 7841-7845. 10.1073/pnas.141221698.View Article
- Reh TA: Neural stem cells: form and function. Nat Neurosci. 2002, 5: 392-394. 10.1038/nn0502-392.View ArticlePubMed
- Black IB, Woodbury D: Adult rat and human bone marrow stromal stem cells differentiate into neurons. Blood Cells Mol Dis. 2001, 27: 632-636. 10.1006/bcmd.2001.0423.View ArticlePubMed
- Chambon P: A decade of molecular biology of retinoic acid receptors. FASEB J. 1996, 10: 940-954.PubMed
- Gottlieb DI, Huettner JE: An in vitro pathway from embryonic stem cells to neurons and glia. Cells Tissues Organs. 1999, 165: 165-172. 10.1159/000016696.View ArticlePubMed
- Lu P, Blesch A, Tuszynski MH: Induction of bone marrow stromal cells to neurons: differentiation, transdifferentiation, or artifact?. J Neurosci Res. 2004, 77: 174-91. 10.1002/jnr.20148.View ArticlePubMed
- Lee ST, Jang JH, Cheong JW, Kim JS, Maemg HY, Hahn JS, Ko YW, Min YH: Treatment of high-risk acute myelogenous leukaemia by myeloablative chemoradiotherapy followed by co-infusion of T cell-depleted haematopoietic stem cells and culture-expanded marrow mesenchymal stem cells from a related donor with one fully mismatched human leucocyte antigen haplotype. Br J Haematol. 2002, 118: 1128-1131. 10.1046/j.1365-2141.2002.03767.x.View ArticlePubMed
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-Phenol reagents. J Biol Chem. 1951, 193: 265-275.PubMed
- Chomczynski P: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 1993, 15: 532-537.PubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.