The effects of different doses of estradiol (E2) on cerebral ischemia in an in vitro model of oxygen and glucose deprivation and reperfusion and in a rat model of middle carotid artery occlusion
- Yu-Long Ma†1,
- Pei Qin†1,
- Yan Li†1, 2,
- Lan Shen2,
- Shi-Quan Wang1,
- Hai-Long Dong1,
- Wu-Gang Hou1Email author and
- Li-Ze Xiong1Email author
© Ma et al.; licensee BioMed Central Ltd. 2013
Received: 25 March 2013
Accepted: 25 September 2013
Published: 9 October 2013
Because neuroprotective effects of estrogen remain controversial, we aimed to investigate the effect of different doses of estradiol (E2) on cerebral ischemia using both in vivo and in vitro experiments.
PC12 cells were cultured at physiological (10 nM and 20 nM) or pharmacological (10 μM and 20 μM) dosages of E2 for 24 hours (h). The results of 5-bromodeoxyuridine (Brdu) incorporation and flow cytometric analysis showed that physiological doses of E2 enhanced cell proliferation and pharmacological doses of E2 inhibited cell proliferation. After the cells were exposed to oxygen and glucose deprivation (OGD) for 4 h and reperfusion for 20 h, the results of 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyl tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release assay, flow cytometric analysis and Western blot analysis showed that physiological doses of E2 enhanced cell viability, reduced cell apoptosis and decreased the expression of pro-apoptotic protein caspase-3. In contrast, pharmacological doses of E2 decreased cell viability and induced cell apoptosis. In vivo, adult ovariectomized (OVX) female rats received continuous subcutaneous injection of different doses of E2 for 4 weeks. Transient cerebral ischemia was induced for 2 h using the middle cerebral artery occlusion (MCAO) technique, followed by 22 h of reperfusion. The results of Garcia test, 2, 3, 5-triphenyltetrazolium chloride (TTC) staining showed that 6 μg/kg and 20 μg/kg E2 replacement induced an increase in neurological deficit scores, a decrease in the infarct volume and a reduction in the expression of caspase-3 when compared to animals in the OVX group without E2 treatment. However, 50 μg/kg E2 replacement treatment decreased neurological deficit scores, increased the infarct volume and the expression of caspase-3 when compared to animals in the control group and 6 up/kg or 20 μg/kg E2 replacement group.
We conclude that physiological levels of E2 exhibit neuroprotective effects on cerebral ischemia; whereas, pharmacological or supraphysiological doses of E2 have damaging effects on neurons after cerebral ischemia.
KeywordsEstrogen Neuroprotection Ischemia Middle carotid artery occlusion (MCAO) Oxygen and glucose deprivation (OGD)
Substantial research efforts have been made to investigate the potential beneficial effects of estrogens on incidence and mortality of stroke. Of these, some studies have shown a neuroprotective effect of estrogen [1, 2], while other studies have not found beneficial effects of estrogen and hormone replacement therapy (HRT) [3, 4]. The largest study, the Women’s Health Initiative (WHI), including more than 16,000 women, was interrupted prematurely because of findings of an increased risk of coronary heart disease, breast cancer and stroke associated with estrogen . Thus, similar clinical studies have been discontinued.
Animal models have been widely applied to elucidate the effects of estrogens on stroke. Dose and route of administration of E2 have become one of primary areas of research focus [5, 6]. Several studies have confirmed that low physiological dose of E2, which are strikingly similar to low-basal circulating levels found in cycling animals, exert profound neuroprotective actions by reducing apoptosis and enhancing proliferation of newborn neurons [7, 8]. Supraphysiological concentrations (Premarin 1 mg/kg IV, plasma estradiol = 171 ± 51 pg/mL) of E2 administered immediately before the onset of ischemic injury have also been shown to exert neuroprotection . Moreover, pharmacological doses (500 and 1000 μg/kg) of E2 effectively protect the brain from ischemic injury when administered as late as 6 h after the onset of brain injury . However, in a recent review, a systematic analysis of 66 studies of effects of estrogens on ischemic brain damage, indicated that E2 increased neurological damage. This effect was suggested to be associated with plasma concentration of pellets with an early, prolonged, supraphysiological peak of estrogen other than the method of induction of ischemic brain lesions, the choice of variables for measurement of outcome, plasma concentration of estrogens at the time of ischemia, and characterization of the rat population such as sex, strain, age, and diseases . Thus, based on these studies, we aimed to systemically investigate the effects of physiological doses of estradiol (E2) and supraphysiological or pharmacological doses of E2 on cerebral ischemia both in vivo and in vitro.
The experiment was conducted both in vitro and in vivo. In vitro experiments, PC12 cells were received physiological and pharmacological doses of estrogen stimulation . Morphological changes of cells were observed by light microscopy, and cell proliferation was detected by Brdu incorporation and flow cytometric analysis. After PC12 cells were exposed to oxygen and glucose deprivation (OGD) for 4 hours (h), the cells were reperfused for 20 h. Cell viability was detected by MTT assay, cell damage was validated by LDH release assay, and cell apoptosis was detected by flow cytometric analysis and western blot. In in vivo experiments, 12 weeks-old female Sprague–Dawley (SD) rats were ovariectomized (OVX), and following a 10-day recovery period, the animals were subjected to a daily subcutaneous injection of different doses of E2 for 4 weeks via an injection on the back of the neck. The animals were then subjected to middle carotid artery occlusion (MCAO). After 2 h of transient occlusion, cerebral blood flow was restored by removing a nylon suture for 22 h. Finally, neurological deficits were assessed by the Garcia test, 2, 3, 5-triphenyltetrazolium chloride (TTC) staining was utilized to evaluate infarct volume. Nissl staining was used to observe the morphologic neuronal changes in ischemic penumbra; and western blot was used to detect apoptosis in ischemic penumbra. All reagents were purchased from Sigma (St Louis, Mo, USA), except those noted to be purchased from other suppliers.
The PC12 cells were plated at a density of 3 × 105cells/well in a 6-well multiwall plate or 104 cells/well in a 96-well multiwall plate at 37°C under 5% CO2 and 95% oxygen in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, streptomycin (100 μg/ml) and penicillin (100 units/mL). The cells were treated with different concentrations of E2 (Cayman, America), which were diluted in dimethyl sulfoxide (DMSO) solution (1:5000). The cells were divided into several groups: group A: negative control; group B: DMSO; group C: 10 nM E2; group D: 20 nM E2; group E: 10 μM E2; and group F: 20 μM E2. After 24 h treatment, the morphology of the cells in the 6-well multiwall plates was observed and recorded using an Olympus Microscope (Tokyo, Japan).
BrdU incorporation assay
Cell proliferation was determined by immunocytochemical assessment of BrdU incorporation into replicating DNA of living cells using the Cellomics BrdU Cell Proliferation kit (Thermo Fisher Scientific, Pittsburgh, PA). Briefly, the PC12 cells were plated at a density of 1.5 × 104 cells/well on glass coverslips in 24-well multiwells and the cells were incubated with 50 μM Brdu with different concentrations of E2, as described above. After 24 h incubation, the cells were fixed with 4% paraformaldehyde for 1 h, followed by permeabilization and blocking. After washing, the sections were probed with mouse anti-BrdU primary antibody (1:500 dilution, Sigma) overnight at 4°C, followed by FITC-conjugated donkey anti-mouse IgG (1:500 dilution, Invitrogen) at room temperature for 45 minutes (min) in the dark. Propidium iodide (PI) dye was used to label all nuclei. The sections were mounted with 50% glycerol for examination under a fluorescence microscope.
Cell cycle analysis
Cell cycle was assessed by flow cytometry, as previously described . After 24 h E2 treatment, the cells were collected by trypsinization, and centrifuged in phosphate buffered saline (PBS) twice. The cells were then fixed in pre-cooled 70% ethanol at -20°C, and stained with PI solution. DNA content was determined by flow cytometry using CellQuest Software. 10,000 events were counted for each sample (FACSCalibur, Becton–Dickinson). The percentage of cells in a particular cell cycle stage was calculated by the ModFit software (Becton–Dickinson, USA).
Oxygen and glucose deprivation and reperfusion (OGD-R)
After 24 h incubation with E2, the cells were washed twice in d-Hanks buffer and switched to d-Hanks buffer (OGD medium) with different E2 concentrations. Then the cells were switched to a modular incubator chamber. The chamber was flushed with 3 L/min of a 95% N2/5% CO2 gas mixture for 30 min at room temperature at 3 L/min. The chamber was then sealed and placed in a 37°C container. OGD was carried out for 4 h. Following the OGD, the cells were incubated with DMEM (without fetal bovine serum) with different E2 concentrations for an additional 20 h reperfusion under normal conditions.
Cell viability analysis
After OGD-R, the MTT assay was used to detect cell viability, as previously described . Briefly, MTT was dissolved in DMEM, and added to each well for incubation at a final concentration of 0.5 mg/ml at 37°C for 4 h. Then, the medium was replaced with 150 μl of DMSO. The optical density (OD) was recorded on a Universal Microplate Reader (Elx 800, Bio-TEK instruments Inc., USA) at 490 nm. Cell viability was expressed as a percentage of the control value.
LDH release assay
After OGD-R, cytotoxicity was quantitatively assessed by measuring the activity of LDH released from the damaged cells into the culture medium. Briefly, cells were treated with 0.5% Triton X-100, and the media which contained detached cells were collected and centrifuged. The supernatant was used for the assay of LDH activity. The enzyme activity was determined by using an assay kit according to the manufacturer’s instructions. LDH leakage was expressed as the percentage of the total LDH activity (LDH in the medium + LDH in the cells), according to the equation LDH released (%) = (LDH activity in the medium/total LDH activity) × 100. Cultures under normal conditions (control group) represent basal LDH release.
Flow cytometric analysis
After OGD-R, cell apoptosis was assayed by flow cytometry, as previously described . Briefly,the cells were washed with 1 × annexin V-FITC binding buffer prior to staining with annexin V-FITC and PI for 15 min at room temperature in the dark. The stained cells were immediately analyzed using flow cytometry. Apoptotic and necrotic cells were quantitated by annexin V binding and PI uptake. The annexin V-FITC+/PI– cell populations were considered to represent apoptotic cells.
One hundred and twenty female adult SD rats weighing 200–220 g were obtained from the Laboratory Animal Center of the Fourth Military Medical University. The animals were maintained under a 12:12-h light–dark cycle and 25°C temperature. The experimental procedures in this study were approved by the Ethics Committee for Animal Experimentation of the Fourth Military Medical University, P. R. China.
OVX and estrogen replacement
The animals were randomly divided into five groups (n = 24): group A: sham control; group B: rats with OVX without E2 replacement; group C: rats with OVX and 6 μg/kg E2 replacement group; group D: rats with OVX and 20 μg/kg E2 replacement group; and group E: rats with OVX and 50 μg/kg E2 replacement group. OVX was adopted by dorsolateral incisions, as previously described . The animals in the sham group were subjected to the same operation; however, their ovaries were kept intact. Five days following OVX, vaginal smears were taken for 5 days before estrogen replacement therapy to confirm OVX and cessation of the estrous cycle . Then these animals received daily subcutaneous injection of different doses of E2 (diluted in sesame oil solution) on the back of the neck for four weeks. The dose of estrogen replacement was based on previous studies  and the results of our pilot study.
Four weeks after HRT, MCAO was conducted, as previously described . The animals within the sham group were confirmed in diestrus by vaginal smears before MCAO. After 2 h of transient occlusion, cerebral blood flow was restored by removing a nylon suture for 22 h. Physiological parameters included rectal temperature, blood pressure, heart rate, blood gas, and glucose were monitored, as previously described .
Detection of serum estrogen level
The levels of serum estrogen were detected to confirm HRT. Briefly, after neurological evaluation, the animals were anesthetized with an overdose of pentobarbital sodium. The blood was collected from the ophthalmic artery. Serum estradiol was measured by EIA kit (Cayman Chemical, Ann Arbor, MI).
Nissl staining was applied to observe morphologic changes in cells within the ischemic penumbra after MCAO. After neurological evaluation, the brains (n = 5) were perfused with cold 4% paraformaldehyde in 0.01 M phosphate-buffered saline (PBS) (pH 7.4). Post-fixation, the brains were taken out and cryoprotected in 20% sucrose and 30% sucrose solution. After the brains were washed in cold water, 14 μm thick sections were prepared using the Leica CM1900 frozen slicer. Then the frozen sections were stained with 0.1% cresyl violet for 20 min, rinsed with PBS, dehydrated by graded alcohol, transparent by xylene, and mounted with neutral gum. The sections were observed using light microscopy.
Assessment of infarct volume
Infarct volume was assessed by TTC staining (n = 13), as previously described . Briefly, after the blood was collected, the rats were decapitated. The brain was rapidly removed and cooled in ice-cold saline for 10 min. Coronal sections (2 mm) were cut and immersed in 2% TTC at 37°C for 30 min, and then transferred to 4% paraformaldehyde in 0.01 M PBS (pH 7.4) for 24 h fixation. The brain slices were photographed using a digital camera (Canon ixus 220HS). The unstained areas were defined as infarcts, and were measured using Photoshop CS3. The infarct volume was calculated by measuring the unstained area in each slice, and multiplying the area by the slice thickness (2 mm). Then the total volume was assessed by summing the area from all six slices.
The expression of pro-apoptotic protein caspase-3 in PC12 cells and ischemic penumbra (n = 6) was detected by Western blotting. In brief, soluble lysates of PC12 cells or penumbra were mixed with sample buffer and NuPAGE reducing agent. Extracted proteins were separated by 12% SDS-PAGE and were then electrically transferred to polyvinylidene difluoride membranes. Afterward, the membranes were blocked in 5% non-fat dry milk diluted in Tris-buffered saline containing 0.1% Tween 20 for 1 h at room temperature. Western blots were probed with rabbit anti-caspase-3 antibodies overnight at 4°C. The membranes were then incubated with an IRDye secondary anti-rabbit antibody for 1 h. Protein bands were visualized using the LI-COR Odyssey System.
Data are presented as means ± SEMs. Differences among the groups were analyzed by one way ANOVA. Differences between two groups were analyzed by two-tailed Student’s t-test with SPSS 11.5. A P-value of < 0.05 was considered statistically significant.
Physiological dose of E2 promote cell proliferation but pharmacological dose of E2 inhibite cell proliferation
In the control and DMSO groups, the percentage of cells in S + G2 phase was 66.3% ± 1.7% and 62.6% ±1.9%, respectively. 10 nM and 20 nM E2 treatment increased the percentage of cells in S + G2 phase to 78.9% ± 5.0% and 75.3% ± 4.8%, respectively (*p < 0.01 v.s. the control and DIMSO group). By contrast, 10 μM and 20 μM E2 treatment decreased the percentage of cells in S + G2 phase to 46.8% ± 2.9% and 42.3% ± 2.7%, respectively (# p < 0.05 v.s. control and DMSO group).
Physiological doses of E2 attenuate cell damage but pharmacological doses of E2 aggregated cell damage
LDH release assay was used to evaluate the effects of E2 on cell necrosis after the cells were exposed to OGD-R (Figure 4B). The level of LDH release in the control group was set as 100% ± 4.7%. The DMSO group was 104.8% ± 4.6%. Results revealed that treatment of 10 nM and 20 nM E2 slightly decreased the level of LDH release to 93.3% ± 5.4% and 90.8 ± 5.1%, respectively. By contrast, 10 μM and 20 μM E2 slightly increased the level of LDH release to 108.1% ± 5.7% and 114.2% ± 3.8%, respectively. However, no significant difference was observed among different doses of E2 treatments and the control and DMSO groups.
Physiological doses of E2 replacement exhibit neuroprotective effects, supraphysiological doses of E2 replacement induced neurodamage
The OVX and estrous cycle were confirmed by vaginal smears as shown in Additional file 1: Figure S1. All rats demonstrated diestrous vaginal smears prior to estradiol treatment, indicating that successful OVX and cessation of the estrous cycle was achieved. Prior to MCAO, the rats in the control group were also confirmed to be on diestrous (Additional file 1: Figure S1).
The OVX and estrogen replacement were further validated by detection of serum estrogen levels. As shown in Additional file 1: Figure S2, the levels of serum estrogen in the control group (19.5 ± 2.5 pg/ml) further indicated that the rats were on diestrous. The level of serum estrogen in OVX group (9.3 ± 1.9 pg/ml) was significantly lower than that in the control group. Compared with the upper limit of serum estrogen level in normal adult rat, the levels of serum estrogen in the rats with 6 μg/kg (17.2 ± 2.7 pg/ml), 20 μg/kg (59.8 ± 12.1 pg/ml) and 50 μg/kg (127.7 ± 25.5 pg/ml) E2 replacement groups successfully mimic relative low, high and supraphysiological level of E2, respectively.
All animals survived 22 h after reperfusion. Physiological parameters of the animals during the MCAO period are summarized in Additional file 1: Table S2. Rectal temperature, blood pressure, heart rate, blood gas, and glucose were remained in the normal range. No significant differences in physiological parameters were observed among groups.
Physiological doses of E2 reduced the expression of caspase-3, supraphysiological doses of E2 increased the the expression of caspase-3
Neuroprotective effects of E2 have been debated for decades [1–4]. Among these, the doses, duration, route and period of estrogen replacement have become the most important parameters. In this study, we demonstrated that in vitro physiological doses of E2 promoted cell proliferation and attenuated cell damage; where zas pharmacological doses of E2 inhibited cell proliferation and promoted cell damage. In vivo experiments further demonstrated that physiological levels of E2 replacement exhibited neuroprotective effects and supraphysiological levels of E2 replacement promoted neurodamage.
Previous reports have demonstrated that estrogen can regulate the development, maturation, survival, and function of multiple types of neurons in different brain regions . However, it is unclear whether the effects of physiological doses of E2 and pharmacological doses of E2 exert different effects on nerve cells. Interestingly, we observed that physiological doses of E2 (10 nM and 20 nM) increased the number of PC12 cells, however, pharmacological doses of E2 (10 μM and 20 μM) decreased the number of cells and induced the cells to lose their neurites. Mechanisms underlying this phenomenon is unclear. Previous research has reported that 0.5 nM E2 was ineffective in promoting proliferation of human neural progenitor cell (hNPC), as assessed by BrdU incorporation. Minimal effectiveness of E2 to promote proliferation was shown at 1 nM, maximal effectiveness at 100 nM and decrement at 250 nM, suggesting that the efficacy of E2 on proliferation was dose dependent . However, the effect of pharmacological doses of E2 on the proliferation of nerve cells has been rarely studied. To explore this question, PC12 cells received different concentrations E2 stimulus. The results of immunocytochemistry demonstrated that physiological doses of E2 could promote cell proliferation, which was consistent with previous findings . Additionally, Suzuki et al. has shown that physiological low doses of E2 protected neurons from brain ischemic injury by enhancing proliferation of newborn neurons. More importantly, our results have demonstrated, for the first time, that pharmacological doses of E2 inhibited the proliferation of nerve cells.
It is known that cell proliferation is determined by the DNA replication during the cell cycle, thus, flow cytometric analysis was used to detect the effects of E2 on the cell cycle. The results revealed that physiological doses of E2 significantly increased the percentage of cells in S + G2 phase of the cell cycle; however, pharmacological doses of E2 markedly decreased percentage of cell in S phase or S + G2 phase. In a previous study, 100 nM E2 has been shown to increase the expression of PCNA and CDK1/cdc2 in hNPCs , PCNA and CDK1/cdc2 are well defined and commonly used as markers of cell cycle. PCNA is a marker of the cells in early G1 phase and S phase of the cell cycle, and it acts as a homotrimer to increase the processing of leading strand synthesis during DNA replication . Whereas CDK1 exists as a component of CDK1/cyclin B complex, is required for transition from G2 to M phase . Taken together, we conclude that physiological doses of E2 promote DNA replication by acting on the PCNA and CDK1/cdc2 complexes. By contrast, it is possible that pharmacological doses of E2 attenuate DNA replication, however, the mechanism is still unclear.
To further investigate the effects of E2 on nerve cells after nerve injury, we applied an OGD-R experimental paradigm. OGD-R is composed of a hypoxic and a reoxygenation/reperfusion phase and it is recognized as an ideal in vitro model for the ischemic stroke. Our results demonstrated that physiological doses of E2 significantly increased cell apoptosis induced by OGD-R. Whereas pharmacological doses of E2 markedly attenuated cell apoptosis. Previous study have shown that 100 nM E2 was found to have a neuroprotective effect against CoCl2-induced apoptosis in PC12 cells by attenuating ROS production and modulating apoptotic signal pathway through caspase cascades, Bcl-2 family, cytochrome c, Fas/Fas-L as well as PI3K/Akt pathway . Studies have reported that chronic E2 treatment could rescue neurons destined to apoptosis or necrosis by interfering with apoptotic death cascades that activate caspase-3 . Our results demonstrated that physiological doses of E2 significantly reduced the expression of pro-apoptotic protein caspase-3, however, pharmacological doses of E2 increased the expression of caspase-3. These results verified that physiological doses of E2 exhibit the neuroprotective effects by modulating apoptotic signal pathway, whereas pharmacological doses of E2 may inhibit the pathway, but the mechanism need to be further explored.
We further examined the effects of different levels of E2 replacement in vivo on OVX rats following MCAO. We found that estrogens used in all previous studies of the neurodamaging effects of E2 were commercially manufactured slow-release pellets, which created an early, prolonged, supraphysiological peak plasma concentration . Therefore, we executed hormone replacement by daily subcutaneous injection of E2 on the back of the neck of the rats for 4 weeks, resulting in the tailored serum E2 level. The result showed that physiological levels of E2 replacement significantly improved neurological deficit scores, decreased infarct volume, reduced neuronal damage and inhibited the apoptosis in ischemic penumbra. However, superphysiological levels of E2 replacement worsen the ischemia–reperfusion injury by significantly lowering neurological deficit scores, increasing infarct volume, inducing neuronal damage and promoting the apoptosis in ischemic penumbra. Several studies [7, 8] have confirmed that low physiological levels of E2, which are strikingly similar to low-basal circulating levels found in cycling animals, exert profound neuroprotective actions by reducing apoptosis, enhancing proliferation of newborn neurons. Moreover, in the studies using commercial pellets, lower doses of estrogen tended to decrease ischemic damage, whereas higher doses tended to increase the damage . Furthermore, a recent study  showed that injection of estrogens into adult OVX female rats 30 mins before conditioning, the low physiological doses of 17β-estradiol and 17a-estradiol enhanced, whereas the superphysiological doses of 17β-estradiol and 17a-estradiol impaired, contextual fear conditioning, which relies on the integrity of the hippocampus and amygdala.
Nevertheless, some studies have shown neuroprotective effects of supraphysiological or pharmacological levels of E2. It has been shown that supraphysiological or pharmacological levels of E2 administered immediately before the onset , or as late as 6 h after the onset , of ischemic injury effectively protected the brain against ischemic injury. It appears that supraphysiological or pharmacological levels of E2 have both neuroprotective effects and neurodamaging effects depending on the timing of E2 administration. These studies [9, 10] reported that acutely administration of E2 but not long-term E2 replacement produced the neuroprotective effects of supraphysiological or pharmacological doses of E2. It is known that estradiol acts through different estrogen receptors (ERs) and activates distinct secondary messenger pathways at different time courses and involves various downstream mechanisms . In the classical chronic genomic mechanism, estradiol acts through soluble intracellular α or β receptors (ERα or ERβ), once these receptors were activated, they translocate to the nucleus where they function as ligand-dependent transcription factors . In contrast, fast non-genomic effects are mediated by classic receptors (ERα and ERβ) and specific G-protein–coupled receptors (GPR30 and ER-X) that regulate ligand-gated ion channels and neurotransmitter transporters . The GPR30 receptor is reported to be a novel estrogen receptor uniquely localized to the endoplasmic reticulum  and may act together with intracellular estrogen receptors to activate cell-signaling pathways to promote neuron survival after global ischemia [34, 35]. In a culture of cortical neurons, treatment with the GPR30 agonist G1 for 45 min attenuated the excitotoxicity induced by NMDA exposure. Additionally, acute neuroprotection mediated by GPR30 is dependent on rapid G-protein–coupled signals and ERK1/2 activation but independent of transcription or translation . Moreover, acute estradiol treatment protects CA1 neurons from ischemia-induced apoptotic cell death via the PI3K/Akt pathway . Therefore, the acute neuroprotective effects of estrogen maybe mediated via the fast non-genomic mechanism. Our results indicated that chronic supraphysiological doses of E2 replacement may exert neuroprodamaging effects, but during acute treatment for ischemic stroke, the supraphysiological or pharmacological doses of E2 may exert neuroprotective effects.
It is worthwhile to note several limitations in this study. First, we had not thoroughly investgate the molecular mechanisms underlying the effects of different doses of E2 on cell morphology, cell proliferation and cell apoptosis. Further studies are warranted to elucidate the mechanisms underlying the attenuation of cell proliferation and increase in cell apoptosis induced by pharmacological doses of E2. Second, the latest study has accurately demonstrated that E2 level in the hippocampus is approximately 8 nM in the male and 0.5–2 nM in the female, which is much higher than that in the circulation . This hippocampus-derived estrogen rapidly modulates dendritic spines , which has been extensively studied in relation to memory processes and synaptic plasticity. Therefore, the effects of estrogen replacement on the changes of estrogen level in the hippocampus should be further studied.
Taken together, physiological levels of E2 exhibit powerful neuroprotective actions both in vivo and in vitro, and these protective actions involve induction of cell proliferation and attenuation of neuronal apoptosis in response to ischemic brain injury. Our results also demonstrated that the supraphysiological levels of E2 attenuate neuroprotective actions in vivo. In vitro experiments demonstrated that high-dose E2 showed neurodamaging effects by attenuating cell proliferation and increasing cell apoptosis. These results may provide a therapeutic basis for clinicians to treat menopausal or ovariectomized women with estrogen replacement therapy.
3-(4 5-dimethylthiazol-2-yl) 2, 5-diphenyl tetrazolium bromide
Middle cerebral artery occlusion
2, 3, 5-triphenyltetrazolium chloride
Oxygen and glucose deprivation and reperfusion
Hormone replacement therapy
Human neural progenitor cell
Proliferating cell nuclear antigen.
This study was supported by the Natural Science Foundation of China (2010: No.81000563; 2011: No. 81100764; 2013: No. 81371446). We thank Medjaden Bioscience Limited for assisting in the preparation of this manuscript.
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