Biogenic amines and their metabolites are differentially affected in the Mecp2-deficient mouse brain
© Panayotis et al; licensee BioMed Central Ltd. 2011
Received: 24 March 2011
Accepted: 24 May 2011
Published: 24 May 2011
Rett syndrome (RTT, MIM #312750) is a severe neurological disorder caused by mutations in the X-linked methyl-CpG binding protein 2 (MECP2) gene. Female patients are affected with an incidence of 1/15000 live births and develop normally from birth to 6-18 months of age before the onset of deficits in autonomic, cognitive, motor functions (stereotypic hand movements, impaired locomotion) and autistic features. Studies on Mecp2 mouse models, and specifically null mice, revealed morphological and functional alterations of neurons. Several functions that are regulated by bioaminergic nuclei or peripheral ganglia are impaired in the absence of Mecp2.
Using high performance liquid chromatography, combined with electrochemical detection (HPLC/EC) we found that Mecp2-/y mice exhibit an alteration of DA metabolism in the ponto-bulbar region at 5 weeks followed by a more global alteration of monoamines when the disease progresses (8 weeks). Hypothalamic measurements suggest biphasic disturbances of norepinephrine and serotonin at pathology onset (5 weeks) that were found stabilized later on (8 weeks). Interestingly, the postnatal nigrostriatal dopaminergic deficit identified previously does not parallel the reduction of the other neurotransmitters investigated. Finally, dosage in cortical samples do not suggest modification in the monoaminergic content respectively at 5 and 8 weeks of age.
We have identified that the level of catecholamines and serotonin is differentially affected in Mecp2-/y brain areas in a time-dependent fashion.
Neither catecholamines nor serotonin levels are affected in the cortex of Mecp2-/y
The alteration of norepinephrine and serotonin levels in the hypothalamus of Mecp2-/y is postnatal and biphasic
Next, we measured biogenic amines concentrations in the hypothalamus. It was previously reported that a targeted deletion of Mecp2 in Single-minded 1 gene (Sim1)-expressing neurons (located in several hypothalamic nuclei) results in a body of behavioural deficits ranging from feeding disturbances to stress . Increased levels of corticosterone and leptin together with Brain-derived neurotrophic factor (Bdnf) deficits are possible contributors for this phenotype in this mouse. Since catecholamines play a role in hypothalamic nuclei neurotransmission and interplays with leptin and Bdnf [14–17] we wanted to assess deficits in bioamines and their degradation products. Deficits were observed at P35 for NE and 5-HT (p < 0.05). No alteration of DA levels was noticed at this age (p > 0.05). However, the NE and 5-HT decrease identified at P35 was no longer present at P55 and DA levels were normal at P55 (p > 0.05) (Figure 2B). All the tested catabolites were found to be normal in the hypothalamus of Mecp2-/y compared to WT at P35 and P55 (Figure 3B).
Bioaminergic disturbances are global and progressive in pontic and brainstem area of Mecp2-/y
DA, NE and 5-HT contents were evaluated by HPLC using pons and brainstem samples of Mecp2-/y animals and WT animals at P35 and P55 (Figure 2C). DA is reduced both at P35 and at P55 (p < 0.05). NE levels are normal at P35 (p > 0.05) but are clearly reduced at P55 (p < 0.05). For serotonin, we found the same pattern of alteration, with no decrease at P35 (p > 0.05) but a decrease at P55 (p < 0.05). Altogether, our results suggest that Mecp2-/y mice have lower levels of biogenic amines than their WT littermates.
We respectively assessed the level of HVA, DOPAC and 5-HIAA in these samples (Figure 3C). At P35, the only catabolite affected is HVA (p < 0.05) whereas DOPAC and 5-HIAA are normal (p > 0.05). At P55, the levels of HVA, DOPAC and 5-HIAA were all reduced in Mecp2-/y compared to WT samples (p < 0.05).
Mecp2-/y nigrostriatal dopaminergic deficits do not parallel noradrenergic or serotonergic ones
We previously reported a postnatal reduction of DA and HVA but not DOPAC in the caudate-putamen of Mecp2-/y at P35 and P55  (illustrated on Figure 2D and Figure 3D). Here, we observed that at P35, neither NE nor 5-HT contents were affected (p > 0.05). This observation stands true for these neurotransmitters at P55 (p > 0.05). We measured the level of 5-HIAA and found no alteration at P35 (p > 0.05), and P55 (p > 0.05). These results indicate that the noradrenergic and serotonergic neurotransmitters are unaffected in the caudate-putamen of Mecp2-/y compared to their WT littermates at all ages investigated (Figure 2D and Figure 3D).
Our previous work failed to detect changes in the level of DA and DOPAC in the Mecp2-/y midbrain at both P35 and P55. However, we observed a clear reduction of HVA in these dopaminergic neurons at the most advanced age  (illustrated on Figure 2E and Figure 3E). We extended our analysis to NE, 5HT and 5-HIAA at both ages. Our results show that NE is not altered at P35 in Mecp2-deficient midbrain (p > 0.05). 5-HT levels are also preserved compared to WT level at the same age (p > 0.05). Samples obtained from symptomatic mice (P55) do no exhibit any modification of the substrate levels compared to age-matched WT (p > 0.05) (Figure 2E). 5-HIAA values are normal at P35 (p > 0.05) and at P55 (p > 0.05) in Mecp2-/y samples compared to age-matched WT (Figure 3E).
Mecp2-/y cortical neurons exhibit several alterations, ranging from reduced activity to abnormal dendritic spine morphology [18, 19]. These defects are influenced by Bdnf dosage . Bdnf plays a key role in neuronal survival, differentiation and synaptic plasticity . Bdnf metabolism was shown to be involved in the development and function of catecholaminergic neurons, affected in Mecp2-/y [22–25]. Recently, several studies identified NE reductions in the piriform cortex of Mecp2-null mouse at P35  and in the prefrontal and motor cortex at both 3 (P21) and 8 (P56) weeks of age . We were not able to reproduce these findings. One possible explanation for this divergence could be differences in the sampling procedure. We performed micropunches of the motor cortex. Different cortical territories receive monoaminergic inputs from different nuclei, some areas being less densely innervated than others . Serotonergic dosage in Mecp2-/y brains were also reported and discrepancies are existing. Isoda and collaborators did not to identify any modifications of 5-HT and its main catabolite 5-HIAA in the prefrontal cortex from P14 to P56 . However, another laboratory argued for a reduction in the same structure of 5-HT only, at 3 and 8 weeks of age . As Isoda and coauthors measured, we did not found significant reductions of 5-HT or 5-HIAA. Another group reported a 5-HT deficit in whole brain extracts in the same developmental window . Unfortunately, samples used in this study came from pooled 6 to 8 weeks Mecp2-/y animals, two ages that are know to be phenotypically different according to the postnatal development of the Mecp2-/y mouse pathology . These results thus combine 5-HT contents from both mildly and severely affected animals.
It is generally believed that catecholamines play a role in hypothalamic nuclei neurotransmission and that their release is modulated by several factors including Bdnf and leptin . Here, we report a postnatal alteration of hypothalamic NE and 5-HT contents that follows a biphasic scheme. These biogenic amines were found significantly decreased at P35 in Mecp2-/y animals whereas their levels are not significantly different from WT at P55. A recent study showed that specific deletion of Mecp2 in Sim1-positive neurons in the paraventricular nucleus (PVN) of the hypothalamus results in higher serum level of leptin . Since leptin was proposed to lower NE-dependant oxytocin release in the PVN  and to reduce firing of serotonergic neurons  it was tempting to assess the level of bioamines in this brain area. Our results at P35 could reflect a leptin-dependant bioaminergic reduction. However, Fyffe and coauthors did not identify alterations at 6 weeks (P42) but noticed a significant increase 42 weeks. It is important to stress that Mecp2-deletion is not restricted to hypothalamic neurons in the model we used. Moreover, mice used in one study were of mixed 129/FVB genetic background  while we used C57Bl6 Mecp2-/y mice .
Metabolite/5-HT and metabolite/DA ratios in the cortex, hypothalamus, caudate-putamen, midbrain and brainstem/pons of WT and Mecp2-/y.
(HVA + DOPAC)/DA ratio
1.68 (+/- 0.14)
1.71 (+/- 0.42)
1.88 (+/- 0.29)
1.33 (+/- 0.43)
1.50 (+/- 0.17)
0.75 (+/- 0.10)
1.47 (+/- 0.29)
0.77 (+/- 0.12)
1.92 (+/- 0.26)
0.85 (+/- 0.06)
2.61 (+/- 0.37) *
0.94 (+/- 0.12)
1.37 (+/- 0.12)
0.73 (+/- 0.15)
1.54 (+/- 0.22)
0.62 (+/- 0.09)
1.91 (+/- 0.11)
0.47 (+/- 0.03)
2.64 (+/- 0.22) **
0.62 (+/- 0.05)
1.06 (+/- 0.13)
0.30 (+/- 0.02)
1.02 (+/- 0.08)
0.33 (+/- 0.02)
1.51 (+/- 0.23)
1.66 (+/- 0.14)
1.79 (+/- 0.27)
1.64 (+/- 0.09)
1.06 (+/- 0.13)
1.07 (+/- 0.13)
1.02 (+/- 0.08)
0.99 (+/- 0.04)
Brainstem + Pons
2.27 (+/- 0.39)
0.36 (+/- 0.03)
2.18 (+/- 0.31)
0.43 (+/- 0.09)
1.17 (+/- 0.06)
0.44 (+/- 0.06)
2.00 (+/- 0.51) *
0.39 (+/- 0.02)
Mecp2-/y mice display a progressive and postnatal alteration in their motor behavior [11, 34]. Interestingly, mice with a targeted deletion of Mecp2 in Th-expressing neurons show a deficit in motricity among other dysregulations . We recently described in mice harboring a constitutive deletion of Mecp2 progressive alterations of the SNpc and the nigrostriatal dopaminergic pathway, a key component of the brain motor circuitry . Moreover, some of the behaviors impaired were ameliorated by L-Dopa, arguing for a strong, although clearly non-unique, involvement of the DA disturbances in Mecp2-/y motor phenotype. In complement to this previous study, we here pursued our neurochemical investigations for NE and 5-HT in the SNpc and its striatal target. NE, provided from the Locus Coeruleus, A1 and A2 (from brainstem) is implicated in several brain functions and its action on midbrain DA neurons was shown to modulate motivational behaviors [35, 36]. The absence of NE and 5-HT disturbances in the midbrain and the caudate-putamen confirm previous findings [10, 11].
The consequence of Mecp2-deficiency on the integrity of dopaminergic and serotonergic nuclei could be indexed on the HVA + DOPAC/DA and 5-HIAA/5-HT ratios respectively since a toxic alteration of dopaminergic and serotonergic systems induces counteradaptative processes that impact on this values [37, 38]. The calculated ratios are reported in Table 1. Results suggest dopamine turnover was left unaffected in all the structures investigated at both P35 and P55 in Mecp2-/y compared to their WT littermates. Conversely, hypothalamus and caudate-putamen dosage revealed a significant increase of 5-HIAA/5-HT ratio at P35 but not P55. Values from the pons and brainstem indicate a clear increase in the serotonergic turnover in Mecp2-/y at P55. However, this index was not modified in the cortex and the midbrain at P35 and P55. Alteration of the serotonergic innervation lead to an increase in the turnover . Interestingly, a reduction in 5-HT immunoreactive fibers was reported in the hippocampus of Mecp2-/y animals . It remains to be elucidated if such phenomenon contribute to serotonergic deficits described here.
Experiments were performed on the B6.129P2(c)-Mecp2tm1-1Bird mouse model for RTT . The mice were obtained from the Jackson Laboratories and maintained on a C57Bl/6 background. The experimental procedures were carried out in keeping with the European guidelines for the care and use of laboratory animals (Council Directive 86/609/EEC). Both pre-symptomatic and symptomatic mice were analyzed at different developmental stages. A total number of 15 Mecp2-/y and 18 WT male mice were used in the study. The Mecp2 -/y (null male) and WT (wild-type male) mice were studied at postnatal days 35 (P35; n = 10 WT; n = 6 Mecp2-/y) and 55 (P55; n = 8 WT; n = 9 Mecp2-/y) for neurochemical analysis. Mecp2-deficient mice were compared to their respective WT littermates of the same gender. Breeding and genotyping were performed as previously described .
P35 (n = 10 WT; n = 6 Mecp2-/y) and P55 (n = 8 WT; n = 9 Mecp2-/y) mice were killed by cervical dislocation, and their brains were dissected out within the first 2 min post-mortem. The motor cortex, Caudate-Putamen, Midbrain area (Substantia Nigra + Ventral Tegmental Area) were microdissected using a punching needle (0.5 mm ∅) and kept at -80°C until biochemical analysis. The region containing both the Pons and the Brainstem was dissected under a binocular microscope (Figure 1). Briefly, brain area dissection was performed on cryostat brain sections with the help of a 5× magnifying lens, following their stereotaxic coordinates [39, 40].
Norepinephrine, DHBA, dopamine, DOPAC, HVA, serotonin (5-hydroxytryptamine, 5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), 1-octanesulfonic acid (OSA), triethylamine and ethylene-diamine-tetra-acetic acid (EDTA) disodium salt were purchased from Sigma, sodium dihydrogen phosphate and citric acid from Merck and methanol from Prolabo. Ultrapure water was obtained with a Milli-Q system (Millipore, Bedford, MA, USA). Standard solutions of each monoamine or metabolite were stored at -20°C at 1 mmol/L as aliquots.
The HPLC system was composed of a Hitachi L-7000 series equipped with a degasser, a L-7100 pump, an L-7200 autosampler thermostated at 10°C and a Decade Intro electrochemical detector fitted with a 3 mm glass carbon working electrode, an Ag/AgCl reference electrode and a 25 μm spacer (Antec, Leyden, The Netherlands). Separations were performed using a 250 mm × 4.6 mm i.d. C18 5 μm Beckman Ultrasphere column equipped with two Phenomenex C18 filters in a security guard system. The mobile phase was pumped at a microflow rate of 0.8 mL/min and composed of 0.7 mol/L sodium phosphate, 0.1 mmol/L EDTA, 1.1 mmol/L OSA, 3.1 mmol/L triethylamine, 14% methanol, pH adjusted to 3.12 with 1 mmol/L citric acid, it was filtered with 0.45 μm cellulose acetate membranes before use. Elutes were detected at an oxidation potential of 700 mV versus reference electrode. The column and the detection cell were housed within the Faraday cage of the electrochemical detector that was set to 25.5°C. The day of the analysis, 35 μL samples were placed in the autosampler and kept at +10°C before injection. The injection volume was 30 μL. The retention times were 7 min, 11 min, 13.5 min, 16.5 min, 21 min, 32 min and 42 min for NE, DHBA, DOPAC, DA, 5-HIAA, HVA, and 5-HT, respectively.
We evaluated whether our data distribution fitted with a Gaussian representation using a K-S Kolmogorov-Smirnov Normality test. If valid, data were statistically analyzed using unpaired Student's t test. If not valid, we used an adapted non-parametric Mann-Whitney test to compare genotypes. The results are reported as mean +/- standard error of the mean (S.E.M). A p-value < 0.05 was considered to be statistically significant.
This study was supported by Inserm, the Provence-Alpes-Côte d'Azur region, AFSR (Association Française du Syndrome de Rett), the E-rare EuroRett network, the Fondation Jérôme Lejeune and the Dischrom project (FP7). Thanks are due to Dr. Sandrine Parrot and Dr. Luc Denoroy from the NeuroChem facility (Université Claude Bernard, Lyon, France) for technical assistance on high performance liquid chromatography.
- Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY: Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. 1999, 23 (2): 185-188. 10.1038/13810.PubMedGoogle Scholar
- Laurvick CL, de Klerk N, Bower C, Christodoulou J, Ravine D, Ellaway C, Williamson S, Leonard H: Rett syndrome in Australia: a review of the epidemiology. J Pediatr. 2006, 148 (3): 347-352. 10.1016/j.jpeds.2005.10.037.PubMedGoogle Scholar
- Chahrour M, Zoghbi HY: The story of Rett syndrome: from clinic to neurobiology. Neuron. 2007, 56 (3): 422-437. 10.1016/j.neuron.2007.10.001.PubMedGoogle Scholar
- Hagberg B, Hanefeld F, Percy A, Skjeldal O: An update on clinically applicable diagnostic criteria in Rett syndrome. Comments to Rett Syndrome Clinical Criteria Consensus Panel Satellite to European Paediatric Neurology Society Meeting, Baden Baden, Germany, 11 September 2001. Eur J Paediatr Neurol. 2002, 6 (5): 293-297. 10.1053/ejpn.2002.0612.PubMedGoogle Scholar
- Neul JL, Kaufmann WE, Glaze DG, Christodoulou J, Clarke AJ, Bahi-Buisson N, Leonard H, Bailey ME, Schanen NC, Zappella M, Renieri A, Huppke P, Percy AK: Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol. 2010, 68 (6): 944-950. 10.1002/ana.22124.PubMed CentralPubMedGoogle Scholar
- Ide S, Itoh M, Goto Y: Defect in normal developmental increase of the brain biogenic amine concentrations in the mecp2-null mouse. Neurosci Lett. 2005, 386 (1): 14-17. 10.1016/j.neulet.2005.05.056.PubMedGoogle Scholar
- Samaco RC, Mandel-Brehm C, Chao HT, Ward CS, Fyffe-Maricich SL, Ren J, Hyland K, Thaller C, Maricich SM, Humphreys P, Greer J, Percy A, Glaze D, Zoghbi HY, Neul JL: Loss of MeCP2 in aminergic neurons causes cell-autonomous defects in neurotransmitter synthesis and specific behavioral abnormalities. Proc Natl Acad Sci USA. 2009Google Scholar
- Roux JC, Villard L: Biogenic Amines in Rett Syndrome: The Usual Suspects. Behav Genet. 2009, 40: 59-75.PubMedGoogle Scholar
- Taneja P, Ogier M, Brooks-Harris G, Schmid DA, Katz DM, Nelson SB: Pathophysiology of locus ceruleus neurons in a mouse model of Rett syndrome. J Neurosci. 2009, 29 (39): 12187-12195. 10.1523/JNEUROSCI.3156-09.2009.PubMed CentralPubMedGoogle Scholar
- Isoda K, Morimoto M, Matsui F, Hasegawa T, Tozawa T, Morioka S, Chiyonobu T, Nishimura A, Yoshimoto K, Hosoi H: Postnatal changes in serotonergic innervation to the hippocampus of methyl-CpG-binding protein 2-null mice. Neuroscience. 2010, 165 (4): 1254-1260. 10.1016/j.neuroscience.2009.11.036.PubMedGoogle Scholar
- Santos M, Summavielle T, Teixeira-Castro A, Silva-Fernandes A, Duarte-Silva S, Marques F, Martins L, Dierssen M, Oliveira P, Sousa N, Maciel P: Monoamine deficits in the brain of methyl-CpG binding protein 2 null mice suggest the involvement of the cerebral cortex in early stages of Rett syndrome. Neuroscience. 2010, 170 (2): 453-467. 10.1016/j.neuroscience.2010.07.010.PubMedGoogle Scholar
- Panayotis N, Pratte M, Borges-Correia A, Ghata A, Villard L, Roux JC: Morphological and functional alterations in the substantia nigra pars compacta of the Mecp2-null mouse. Neurobiol Dis. 2011, 41 (2): 385-397. 10.1016/j.nbd.2010.10.006.PubMedGoogle Scholar
- Fyffe SL, Neul JL, Samaco RC, Chao HT, Ben-Shachar S, Moretti P, McGill BE, Goulding EH, Sullivan E, Tecott LH, Zoghbi HY: Deletion of Mecp2 in Sim1-expressing neurons reveals a critical role for MeCP2 in feeding behavior, aggression, and the response to stress. Neuron. 2008, 59 (6): 947-958. 10.1016/j.neuron.2008.07.030.PubMed CentralPubMedGoogle Scholar
- Berridge CW: Neural substrates of psychostimulant-induced arousal. Neuropsychopharmacology. 2006, 31 (11): 2332-2340. 10.1038/sj.npp.1301159.PubMedGoogle Scholar
- Khan AM, Ponzio TA, Sanchez-Watts G, Stanley BG, Hatton GI, Watts AG: Catecholaminergic control of mitogen-activated protein kinase signaling in paraventricular neuroendocrine neurons in vivo and in vitro: a proposed role during glycemic challenges. J Neurosci. 2007, 27 (27): 7344-7360. 10.1523/JNEUROSCI.0873-07.2007.PubMedGoogle Scholar
- Rodriguez Fermepin M, Trinchero M, Minetto J, Beltran A, Fernandez BE: Brain derived neurotrophic factor and neurotrophin-4 employ different intracellular pathways to modulate norepinephrine uptake and release in rat hypothalamus. Neuropeptides. 2009, 43 (4): 275-282. 10.1016/j.npep.2009.06.001.PubMedGoogle Scholar
- Kutlu S, Aydin M, Alcin E, Ozcan M, Bakos J, Jezova D, Yilmaz B: Leptin modulates noradrenaline release in the paraventricular nucleus and plasma oxytocin levels in female rats: a microdialysis study. Brain Res. 2010, 1317: 87-91.PubMedGoogle Scholar
- Dani VS, Chang Q, Maffei A, Turrigiano GG, Jaenisch R, Nelson SB: Reduced cortical activity due to a shift in the balance between excitation and inhibition in a mouse model of Rett syndrome. Proc Natl Acad Sci USA. 2005, 102 (35): 12560-12565. 10.1073/pnas.0506071102.PubMed CentralPubMedGoogle Scholar
- Tropea D, Giacometti E, Wilson NR, Beard C, McCurry C, Fu DD, Flannery R, Jaenisch R, Sur M: Partial reversal of Rett Syndrome-like symptoms in MeCP2 mutant mice. Proc Natl Acad Sci USA. 2009, 106 (6): 2029-2034. 10.1073/pnas.0812394106.PubMed CentralPubMedGoogle Scholar
- Chang Q, Khare G, Dani V, Nelson S, Jaenisch R: The disease progression of Mecp2 mutant mice is affected by the level of BDNF expression. Neuron. 2006, 49 (3): 341-348. 10.1016/j.neuron.2005.12.027.PubMedGoogle Scholar
- Poo MM: Neurotrophins as synaptic modulators. Nat Rev Neurosci. 2001, 2 (1): 24-32. 10.1038/35049004.PubMedGoogle Scholar
- Balkowiec A, Katz DM: Brain-derived neurotrophic factor is required for normal development of the central respiratory rhythm in mice. J Physiol. 1998, 510 (Pt 2): 527-533.PubMed CentralPubMedGoogle Scholar
- Erickson JT, Conover JC, Borday V, Champagnat J, Barbacid M, Yancopoulos G, Katz DM: Mice lacking brain-derived neurotrophic factor exhibit visceral sensory neuron losses distinct from mice lacking NT4 and display a severe developmental deficit in control of breathing. J Neurosci. 1996, 16: 5361-71.PubMedGoogle Scholar
- Viemari JC, Roux JC, Tryba AK, Saywell V, Burnet H, Pena F, Zanella S, Bevengut M, Barthelemy-Requin M, Herzing LB, Moncla A, Mancini J, Ramirez JM, Villard L, Hilaire G: Mecp2 deficiency disrupts norepinephrine and respiratory systems in mice. J Neurosci. 2005, 25 (50): 11521-11530. 10.1523/JNEUROSCI.4373-05.2005.PubMedGoogle Scholar
- Oo TF, Marchionini DM, Yarygina O, O'Leary PD, Hughes RA, Kholodilov N, Burke RE: Brain-derived neurotrophic factor regulates early postnatal developmental cell death of dopamine neurons of the substantia nigra in vivo. Mol Cell Neurosci. 2009, 41 (4): 440-447. 10.1016/j.mcn.2009.04.009.PubMed CentralPubMedGoogle Scholar
- Gu Q: Neuromodulatory transmitter systems in the cortex and their role in cortical plasticity. Neuroscience. 2002, 111 (4): 815-835. 10.1016/S0306-4522(02)00026-X.PubMedGoogle Scholar
- Calfa G, Percy AK, Pozzo-Miller L: Experimental models of Rett syndrome based on Mecp2 dysfunction. Exp Biol Med (Maywood). 2011, 236 (1): 3-19. 10.1258/ebm.2010.010261.Google Scholar
- Yadav VK, Oury F, Suda N, Liu ZW, Gao XB, Confavreux C, Klemenhagen KC, Tanaka KF, Gingrich JA, Guo XE, Tecott LH, Mann JJ, Hen R, Horvath TL, Karsenty G: A serotonin-dependent mechanism explains the leptin regulation of bone mass, appetite, and energy expenditure. Cell. 2009, 138 (5): 976-989. 10.1016/j.cell.2009.06.051.PubMed CentralPubMedGoogle Scholar
- Guy J, Hendrich B, Holmes M, Martin JE, Bird A: A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome. Nat Genet. 2001, 27 (3): 322-326. 10.1038/85899.PubMedGoogle Scholar
- Chen RZ, Akbarian S, Tudor M, Jaenisch R: Deficiency of methyl-CpG binding protein-2 in CNS neurons results in a Rett-like phenotype in mice. Nat Genet. 2001, 27 (3): 327-331. 10.1038/85906.PubMedGoogle Scholar
- Katz DM, Dutschmann M, Ramirez JM, Hilaire G: Breathing disorders in Rett syndrome: progressive neurochemical dysfunction in the respiratory network after birth. Respir Physiol Neurobiol. 2009, 168 (1-2): 101-108. 10.1016/j.resp.2009.04.017.PubMed CentralPubMedGoogle Scholar
- Lioy DT, Wu WW, Bissonnette JM: Autonomic dysfunction with mutations in the gene that encodes methyl-CpG-binding protein 2: Insights into Rett syndrome. Auton Neurosci. 2011Google Scholar
- Bonham AC: Neurotransmitters in the CNS control of breathing. Respir Physiol. 1995, 101 (3): 219-230. 10.1016/0034-5687(95)00045-F.PubMedGoogle Scholar
- Pratte M, Panayotis N, Gatha A, Villard L, Roux JC: Progressive motor and respiratory metabolism deficits in post-weaning Mecp2-null male mice. Behav Brain Res. 2011, 216 (1): 313-20. 10.1016/j.bbr.2010.08.011.PubMedGoogle Scholar
- Tassin JP: Uncoupling between noradrenergic and serotonergic neurons as a molecular basis of stable changes in behavior induced by repeated drugs of abuse. Biochem Pharmacol. 2008, 75 (1): 85-97. 10.1016/j.bcp.2007.06.038.PubMedGoogle Scholar
- Sara SJ: The locus coeruleus and noradrenergic modulation of cognition. Nat Rev Neurosci. 2009, 10 (3): 211-223. 10.1038/nrn2573.PubMedGoogle Scholar
- Guevara BH, Torrico F, Hoffmann IS, Cubeddu LX: Lesion of caudate-putamen interneurons with kainic acid alters dopamine and serotonin metabolism in the olfactory tubercle of the rat. Cell Mol Neurobiol. 2002, 22 (5-6): 835-844.PubMedGoogle Scholar
- Youdim MB, Arraf Z: Prevention of MPTP (N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) dopaminergic neurotoxicity in mice by chronic lithium: involvements of Bcl-2 and Bax. Neuropharmacology. 2004, 46 (8): 1130-1140. 10.1016/j.neuropharm.2004.02.005.PubMedGoogle Scholar
- Paxinos G, Franklin K: The Mouse Brain in Stereotaxic Coordinates. 2001, Academic, San DiegoGoogle Scholar
- Roux JC, Mamet J, Perrin D, Peyronnet J, Royer C, Cottet-Emard JM, Pequignot JM, Dalmaz Y: Neurochemical development of the brainstem catecholaminergic cell groups in rat. J Neural Transm. 2003, 110 (1): 51-65.PubMedGoogle Scholar