Newly generated cells are increased in hippocampus of adult mice lacking a serine protease inhibitor
© Lino et al; licensee BioMed Central Ltd. 2010
Received: 27 November 2009
Accepted: 8 June 2010
Published: 8 June 2010
Neurogenesis in the hippocampal dentate gyrus and the subventricular zone occurs throughout the life of mammals and newly generated neurons can integrate functionally into established neuronal circuits. Neurogenesis levels in the dentate gyrus are modulated by changes in the environment (enrichment, exercise), hippocampal-dependent tasks, NMDA receptor (NMDAR) activity, sonic hedgehog (SHH) and/or other factors.
previously, we showed that Protease Nexin-1 (PN-1), a potent serine protease inhibitor, regulates the NMDAR availability and activity as well as SHH signaling. Compared with wild-type (WT), we detected a significant increase in BrdU-labeled cells in the dentate gyrus of mice lacking PN-1 (PN-1 -/-) both in controls and after running exercise. Patched homologue 1 (Ptc1) and Gli1 mRNA levels were higher and Gli3 down-regulated in mutant mice under standard conditions and to a lesser extent after running exercise. However, the number of surviving BrdU-positive cells did not differ between WT and PN-1 -/- animals. NMDAR availability was altered in the hippocampus of mutant animals after exercise.
All together our results indicate that PN-1 controls progenitors proliferation through an effect on the SHH pathway and suggest an influence of the serpin on the survival of newly generated neurons through modulation of NMDAR availability.
Neurogenesis occurs throughout the life of mammals [1, 2] where the hippocampal dentate gyrus and the subventricular zone (SVZ) retain the ability to generate new neurons during adulthood [3, 4]. In the hippocampus, granule neurons are generated from a population of continuously dividing cells residing in the subgranular zone of the dentate gyrus [2, 5, 6]. These "newborn" progenitor cells migrate into the granule cell layer, differentiate, extend axons and express neuronal marker proteins .
Newly generated neurons can integrate functionally into neuronal circuits  and represent a powerful means of brain repair. Neurogenesis in the dentate gyrus can be modulated by enrichment of the environment and by behavior, such as exercise and hippocampal-dependent tasks [4, 9–11]. In particular, voluntary exercise in a running wheel has been shown to be the most efficient mean of increasing hippocampal cell proliferation, cell survival and net neurogenesis [11–13]. In contrast, exposure to acute psychosocial stress results in rapid decline of proliferation in the dentate gyrus [14, 15].
At present, little is known about the mechanisms controlling the generation of new neurons. Neuron generation and survival can be mediated partially by trophic factors  such as brain-derived nerve growth factor (BDNF) [17–20], vascular endothelial growth factor , insulin like growth factor , fibroblast growth factor , SHH  and others. A further mechanism implicated in adult brain neurogenesis is excitatory input and NMDAR activation. Blockade of NMDAR increases proliferation in the dentate gyrus [14, 15] and the overall density of neurons in the granule cell layer . Moreover, it was reported recently that survival of new neurons is regulated by the relative levels of NMDAR activation [26, 27].
Previously, we showed that the serine protease inhibitor PN-1 regulates NMDAR availability, leading to an altered electrophysiology detected so far in the hippocampus and the barrel cortex [28, 29]. Recently we found that PN-1 contributes to shaping of the cerebellum by promoting cell cycle exit through inhibition of SHH signaling .
During embryogenesis and in the postnatal brain, PN-1 is expressed prominently at different times in areas of high remodeling [31, 32]. In particular, the distributions of Shh and PN-1 transcripts overlap in various developing organs. In the developing central nervous system, Shh and PN-1 are co-expressed in the ventral part of the mesencephalon and myelencephalon, the mid-hindbrain junction, cerebellum and optic vesicles . Recently, we showed that PN-1 modulates SHH signalling strength during postnatal development of the cerebellum in mice. In particular, in PN-1 deficient mice, the proliferation of the granular cells neuronal precursors is increased while initiation of their differentiation is delayed. This results in overproduction of mature granular cells and subsequent expansion of regionalized lobes . It was therefore of great interest to investigate whether adult neurogenesis, especially cell proliferation and survival, is affected in the hippocampus of mice lacking PN-1.
PN-1 expression in the dentate gyrus
Cell proliferation in WT and PN-1 -/- mice
SHH pathway activation is influenced by PN-1 expression and running exercise
Survival of newly generated neurons is impaired in PN-1 -/- mice
Different effects of running on NMDAR subunit expression in wt vs. PN-1 -/- animals
Given that NMDAR availability and function are reduced in the hippocampus and the barrel cortex of PN-1 -/- mice [28, 29], we were intrigued by the reports that receptor subunits availability changes after running challenge [9, 15, 33, 34]. Moreover, it was recently reported that NMDAR is needed for the integration of new neurons in the adult dentate gyrus [26, 27].
Our results show that mice deficient for the endogenous protease inhibitor PN-1 have a higher proliferation rate in the hippocampus. However, the newly produced cells do not survive. The increased proliferation rate is mainly associated with a constitutive overactivation of the SHH pathway and abnormal NMDAR availability could account for the altered survival.
SHH is one of the critical regulators of neurogenesis, including proliferation of adult hippocampal neural stem cells in vitro and in vivo [24, 35]. The importance of the SHH signaling pathway has been demonstrated using cyclopamine or ectopic expression of the protein . These authors showed that SHH is not expressed locally in the hippocampus, but anterogradly transported from the basal forebrain via the fornix. Similar to the situation in the developing cerebellum , the increased Gli1 and Ptc1 expression and the decreased Gli3 mRNA levels observed here in the hippocampus of PN-1 -/- mice (Fig. 2) indicate an enhancement of the SHH signaling pathway. Our results show for the first time that running exercise triggers the hippocampal SHH pathway. The stimulation is weaker in PN-1 -/- than in WT mice. This is probably due to a high preexisting basal level of SHH signaling evidenced by increased Gli1 and Ptc1, respectively decreased Gli3 mRNA levels in the mutant mice.
We cannot exclude that the lower availability of NMDAR detected in mutant mice after running could also contribute to an increase in progenitor cell proliferation. Similar to the responses to enriched environment , 12 days of running exercise led to a decrease in the levels of NR1, NR2A and NR2B NMDAR subunits in PN-1 -/- mice (Fig. 4 A-D). The impact of NMDAR stimulation on hippocampal cell proliferation is still a matter of debate. NMDAR antagonism has been shown to stimulate adult neurogenesis in the dentate gyrus [15, 33, 34, 36], while a recent study supports a stimulatory function upon short-term NMDA signal transduction . The decline in the availability of NMDAR subunits in the mutant mice after running may be functionally similar to the antagonism of NMDAR, thus contributing to the detected increase in proliferation. In fact, as the increase in the already enhanced SHH pathway after exercise is quite marginal in the mutant mice, the impact due to reduced NMDAR activation could be more important in these animals.
We investigated whether the higher number of cells produced in the mutant would lead to an increase in cells able to survive and integrate. In fact, only a small fraction of the newly generated cell population survived in the adult brain. For example, in the hippocampus or the SVZ, 50% of the newborn cells double-labeled with neuron-specific markers  were found to die within 2 months of birth [5, 10, 38–40]. This phenomenon is enhanced in PN-1 -/- mice. The difference in the number of BrdU-positive cells in WT and mutant mice found after 12 days, both with and without exercise (Fig. 2A, B), was not observed 4 weeks later (Fig. 2C,D). Moreover, a 3-D reconstruction did not provide evidence for an increase in size affecting a specific substructure of the dentate gyrus, as detected in the cerebellum of PN-1 -/- mice . The excess of progenitor cells resulting from PN-1 gene deficiency is probably neutralized by altered survival.
This observation is in line with the proposal that NMDAR activation promotes neuronal differentiation and integration [26, 27, 34, 36]. In particular, the NR2B subunit was identified recently as one of the major players in the functionality of newly generated neurons . It is thus interesting to stress that this subunit decreased significantly in the PN-1 mutant following running exercise (Fig. 4D). The reduction may thus explain the observed decrease in neuronal integration in the mutant. Consequently, the failure of PN-1 -/- mice to integrate cells generated during the first 12 days of exercise may also be due to the reduced NMDA-dependent stimulation detected in the cortex and hippocampus of the mutant mice [28, 29].
The results from this study reveal a dual effect of PN-1, which regulates SHH-induced proliferation and may also sustain NMDAR-controlled neuronal integration. In summary, our data identify a PN-1 modulatory function at the crossroad of the SHH and NMDAR signaling pathways, the interplay of which coordinates proliferation versus survival and integration in a neurogenic territory.
Adult WT, knock-in and PN-1-/- mice  were divided into four groups: WT and PN-1-/- mice runners and their controls. The construct used for the knock-in mice (PN-1KI) allows independent translation of PN-1 and β-galactosidase from the transcript . The controls were housed under standard conditions with 3-4 mice per cage. The runners (3-4 animals per cage) were housed for 12 days in a standard cage with one Linton exercise wheel activity counter (model EWAC-R). A different set of animal under the same conditions was used to study NMDAR subunits. C57BL/6 mice were purchased from Charles River (Arbresle, France). All experimental animals were 3 months old.
BrdU (Sigma, St Louis, Missouri) was dissolved in 0.9% Nacl and filtered sterile at 0.2 μm. The mice received single doses of 50 μg/g body weight through one daily intraperitoneal injection of a 10 mg/ml solution, always at the same time for 12 consecutive days . All animal experiments were approved by the Swiss Veterinary Authorities.
For BrdU immunohistochemistry and immunofluorescent triple labeling for BrdU, NeuN and S100b, perfused brains were post-fixed for 12 h in 4% PFA, equilibrated for 24 h in 30% sucrose and quickly frozen in Tissue-Tek O.C.T (Sakura Finetek, USA). Cryosections of 20 μm were mounted on slides to give a series of 6 slides with comparative sections. Prior to antibody incubation, sections were treated in a microwave processing lab-station (Milestone) with citrate buffer, pH 6 for 10 min at 97°C. The primary antibody treatments and the controls were incubated at 4°C. The antibodies used were rat anti-BrdU (Immunologicals Direct, Oxford, UK) 1:300, mouse anti-NeuN (Chemicon, Temecula/CA, USA) 1:1000 and rabbit anti-S100b (Swant, Bellinzona, Switzerland) 1:5000. To detect BrdU-labeled cells. biotinylated goat anti-rat antibody (Vector Laboratories, Burlingham/CA, USA) was applied, followed by the ABC-kit (Vector) and DAB as a substrate. The fluorescent secondary antibodies were goat anti-rat Alexa 488, goat anti-mouse Alexa 594 (Molecular Probes) and goat anti-rabbit Cy5 (Jackson ImmunoResearch). For counting BrdU and triple labeled positive cells we used a Nikon Eclipse E600 microscope equipped with a Leica camera DFC420 with 10 × and 20 × objective, respectively.
Synaptosomal-enriched plasma membrane were prepared as described earlier . Immunoblotting was performed using SDS PAGE and the NuPAGE protein detection systems (Invitrogen) according to the manufacturer's instructions. The antibodies used were anti-NR1 (1:1000) and anti-NR2B (1:500) (UPSTATE Charlottesville, Va.), anti-NR2A (1:500) (Santa Cruz, Santa Cruz, Calif., USA) and anti-actin (1:5000) (NeoMarkers, Fremont, Calif., USA).
One 20-μm section out of four throughout the whole hippocampus were stained and BrdU-positive cells counted on 12 sections covering the entire dentate gyrus. One-way ANOVA with Newman Keul's multiple comparison tests was used for the statistical analysis. Statistical analysis of RT-PCR was performed by t-test assuming unequal variances. Groups of three to four animals were used for each experiment.
Total RNA was prepared using the RNeasy kit (Qiagen) according to the manufacturer's instructions. First-strand cDNA was synthesized using AMV Reverse Transcriptase (Promega) according to the manufacturer's instructions. Aliquots (2 μl) of each cDNA was amplified by PCR with the following primers: Shh: forward: 5'gctgctgctggccagatg 3'; reverse: 5'gttcggagtttcttgagatc 3'; Gli1: forward: 5'tgccagatatgcttcagcca 3', reverse: 5'acctctgtgtctattcgccac 3'; Ptc1: 5'ccaaactccactcaaaaggtgc 3', 5'cattggcaggaggagttgattg 3'; Gli3: forward: 5'gtgccatcgatgaaacc 3'; reverse: 5'ctacgatcccatctccacag 3'. β-actin PCR products were used to normalize the results (forward: 5'gtgggccgctctaggcacaa 3' and reverse: 5'ctctttgatgtcacgcacgatttc 3'). Bands were visualized and quantified by Gene Snap software (Syngene). Thirty cycles were used for Shh, Gli1 and Gli3 detection, 32 cycles were used for Ptc1 and 28 cycles were used for actin detection.
In situ Hybridization
In situ hybridization was performed using 10-μm sagittal brain sections. Gli1, Gli3 and Ptc1 mRNA probes were as described . Three animals per genotype and conditions were used for each experiment. Image-ProPlus sofware was used for the quantification of the in situ signals.
brain-derived nerve growth factor
Patched homologue 1
We thank Sabrina Taieb, Elisabeth Fries, Eliza Moreno and Jean-François Spetz for valuable technical assistance, Botond Roska and Joy Alcedo for advice and critical reading of the manuscript. The editing work by Patrick King is also acknowledged. This research was funded by the Novartis Research Foundation.
- Altman J: Autoradiographic and histological studies of postnatal neurogenesis. IV. Cell proliferation and migration in the anterior forebrain, with special reference to persisting neurogenesis in the olfactory bulb. J Comp Neurol. 1969, 137: 433-457. 10.1002/cne.901370404.View ArticlePubMedGoogle Scholar
- Kaplan MS, Hinds JW: Neurogenesis in the adult rat: electron microscopic analysis of light radioautographs. Science. 1977, 197: 1092-1094. 10.1126/science.887941.View ArticlePubMedGoogle Scholar
- Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, et al.: Neurogenesis in the adult human hippocampus. Nat Med. 1998, 4: 1313-1317. 10.1038/3305.View ArticlePubMedGoogle Scholar
- Kempermann G, Kuhn HG, Gage FH: More hippocampal neurons in adult mice living in an enriched environment. Nature. 1997, 386: 493-495. 10.1038/386493a0.View ArticlePubMedGoogle Scholar
- Cameron HA, Woolley CS, McEwen BS, Gould E: Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat. Neuroscience. 1993, 56: 337-344. 10.1016/0306-4522(93)90335-D.View ArticlePubMedGoogle Scholar
- Stanfield BB, Trice JE: Evidence that granule cells generated in the dentate gyrus of adult rats extend axonal projections. Exp Brain Res. 1988, 72: 399-406.PubMedGoogle Scholar
- Kornack DR, Rakic P: The generation, migration, and differentiation of olfactory neurons in the adult primate brain. Proc Natl Acad Sci USA. 2001, 98: 4752-4757. 10.1073/pnas.081074998.PubMed CentralView ArticlePubMedGoogle Scholar
- Van Praag H, Schinder AF, Christie BR, Toni N, Palmer TD, Gage FH: Functional neurogenesis in the adult hippocampus. Nature. 2002, 415: 1030-1034. 10.1038/4151030a.View ArticlePubMedGoogle Scholar
- Gould E, Beylin A, Tanapat P, Reeves A, Shors TJ: Learning enhances adult neurogenesis in the hippocampal formation. Nat Neurosci. 1999, 2: 260-265. 10.1038/6365.View ArticlePubMedGoogle Scholar
- Van Praag H, Kempermann G, Gage FH: Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus. Nat Neurosci. 1999, 2: 266-270. 10.1038/6368.View ArticlePubMedGoogle Scholar
- Van Praag H, Christie BR, Sejnowski TJ, Gage FH: Running enhances neurogenesis, learning, and long-term potentiation in mice. Proc Natl Acad Sci USA. 1999, 96: 13427-13431. 10.1073/pnas.96.23.13427.PubMed CentralView ArticlePubMedGoogle Scholar
- Arsenijevic Y, Weiss S: Insulin-like growth factor-I is a differentiation factor for postmitotic CNS stem cell-derived neuronal precursors: distinct actions from those of brain-derived neurotrophic factor. J Neurosci. 1998, 18: 2118-2128.PubMedGoogle Scholar
- Van Praag H, Kempermann G, Gage FH: Neural consequences of environmental enrichment. Nat Rev Neurosci. 2000, 1: 191-198. 10.1038/35044558.View ArticlePubMedGoogle Scholar
- Tanapat P, Galea LA, Gould E: Stress inhibits the proliferation of granule cell precursors in the developing dentate gyrus. Int J Dev Neurosci. 1998, 16: 235-239. 10.1016/S0736-5748(98)00029-X.View ArticlePubMedGoogle Scholar
- Gould E, McEwen BS, Tanapat P, Galea LA, Fuchs E: Neurogenesis in the dentate gyrus of the adult tree shrew is regulated by psychosocial stress and NMDA receptor activation. J Neurosci. 1997, 17: 2492-2498.PubMedGoogle Scholar
- Ferguson KL, Slack RS: Growth factors: can they promote neurogenesis?. Trends Neurosci. 2003, 26: 283-285. 10.1016/S0166-2236(03)00100-0.View ArticlePubMedGoogle Scholar
- Adlard PA, Perreau VM, Engesser-Cesar C, Cotman CW: The timecourse of induction of brain-derived neurotrophic factor mRNA and protein in the rat hippocampus following voluntary exercise. Neurosci Lett. 2004, 363: 43-48. 10.1016/j.neulet.2004.03.058.View ArticlePubMedGoogle Scholar
- Adlard PA, Perreau VM, Cotman CW: The exercise-induced expression of BDNF within the hippocampus varies across life-span. Neurobiol Aging. 2005, 26: 511-520. 10.1016/j.neurobiolaging.2004.05.006.View ArticlePubMedGoogle Scholar
- Gomez-Pinilla F, Ying Z, Roy RR, Molteni R, Edgerton VR: Voluntary exercise induces a BDNF-mediated mechanism that promotes neuroplasticity. J Neurophysiol. 2002, 88: 2187-2195. 10.1152/jn.00152.2002.View ArticlePubMedGoogle Scholar
- Oliff HS, Berchtold NC, Isackson P, Cotman CW: Exercise-induced regulation of brain-derived neurotrophic factor (BDNF) transcripts in the rat hippocampus. Brain Res Mol Brain Res. 1998, 61: 147-153. 10.1016/S0169-328X(98)00222-8.View ArticlePubMedGoogle Scholar
- Cao L, Jiao X, Zuzga DS, Liu Y, Fong DM, Young D, et al.: VEGF links hippocampal activity with neurogenesis, learning and memory. Nat Genet. 2004, 36: 827-835. 10.1038/ng1395.View ArticlePubMedGoogle Scholar
- Aberg MA, Aberg ND, Palmer TD, Alborn AM, Carlsson-Skwirut C, Bang P, et al.: IGF-I has a direct proliferative effect in adult hippocampal progenitor cells. Mol Cell Neurosci. 2003, 24: 23-40. 10.1016/S1044-7431(03)00082-4.View ArticlePubMedGoogle Scholar
- Wagner JP, Black IB, DiCicco-Bloom E: Stimulation of neonatal and adult brain neurogenesis by subcutaneous injection of basic fibroblast growth factor. J Neurosci. 1999, 19: 6006-6016.PubMedGoogle Scholar
- Lai K, Kaspar BK, Gage FH, Schaffer DV: Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo. Nat Neurosci. 2003, 6: 21-27. 10.1038/nn983.View ArticlePubMedGoogle Scholar
- Cameron HA, McEwen BS, Gould E: Regulation of adult neurogenesis by excitatory input and NMDA receptor activation in the dentate gyrus. J Neurosci. 1995, 15: 4687-4692.PubMedGoogle Scholar
- Tashiro A, Sandler VM, Toni N, Zhao C, Gage FH: NMDA-receptor-mediated, cell-specific integration of new neurons in adult dentate gyrus. Nature. 2006, 442: 929-933. 10.1038/nature05028.View ArticlePubMedGoogle Scholar
- Tashiro A, Makino H, Gage FH: Experience-specific functional modification of the dentate gyrus through adult neurogenesis: a critical period during an immature stage. J Neurosci. 2007, 27: 3252-3259. 10.1523/JNEUROSCI.4941-06.2007.View ArticlePubMedGoogle Scholar
- Luthi A, Van der PH, Botteri FM, Mansuy IM, Meins M, Frey U, et al.: Endogenous serine protease inhibitor modulates epileptic activity and hippocampal long-term potentiation. J Neurosci. 1997, 17: 4688-4699.PubMedGoogle Scholar
- Kvajo M, Albrecht H, Meins M, Hengst U, Troncoso E, Lefort S, et al.: Regulation of brain proteolytic activity is necessary for the in vivo function of NMDA receptors. J Neurosci. 2004, 24: 9734-9743. 10.1523/JNEUROSCI.3306-04.2004.View ArticlePubMedGoogle Scholar
- Vaillant C, Michos O, Orolicki S, Brellier F, Taieb S, Moreno E, et al.: Protease nexin 1 and its receptor LRP modulate SHH signalling during cerebellar development. Development. 2007, 134: 1745-1754. 10.1242/dev.02840.View ArticlePubMedGoogle Scholar
- Kury P, Schaeren-Wiemers N, Monard D: Protease nexin-1 is expressed at the mouse met-/mesencephalic junction and FGF signaling regulates its promoter activity in primary met-/mesencephalic cells. Development. 1997, 124: 1251-1262.PubMedGoogle Scholar
- Mansuy IM, Van der PH, Schmid P, Meins M, Botteri FM, Monard D: Variable and multiple expression of Protease Nexin-1 during mouse organogenesis and nervous system development. Development. 1993, 119: 1119-1134.PubMedGoogle Scholar
- Kitamura T, Mishina M, Sugiyama H: Enhancement of neurogenesis by running wheel exercises is suppressed in mice lacking NMDA receptor epsilon 1 subunit. Neurosci Res. 2003, 47: 55-63. 10.1016/S0168-0102(03)00171-8.View ArticlePubMedGoogle Scholar
- Nacher J, Rosell DR, Alonso-Llosa G, McEwen BS: NMDA receptor antagonist treatment induces a long-lasting increase in the number of proliferating cells, PSA-NCAM-immunoreactive granule neurons and radial glia in the adult rat dentate gyrus. Eur J Neurosci. 2001, 13: 512-520. 10.1046/j.0953-816x.2000.01424.x.View ArticlePubMedGoogle Scholar
- Palma V, Lim DA, Dahmane N, Sanchez P, Brionne TC, Herzberg CD, et al.: Sonic hedgehog controls stem cell behavior in the postnatal and adult brain. Development. 2005, 132: 335-344. 10.1242/dev.01567.PubMed CentralView ArticlePubMedGoogle Scholar
- Joo JY, Kim BW, Lee JS, Park JY, Kim S, Yun YJ, et al.: Activation of NMDA receptors increases proliferation and differentiation of hippocampal neural progenitor cells. J Cell Sci. 2007, 120: 1358-1370. 10.1242/jcs.002154.View ArticlePubMedGoogle Scholar
- Cameron HA, McKay RD: Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus. J Comp Neurol. 2001, 435: 406-417. 10.1002/cne.1040.View ArticlePubMedGoogle Scholar
- Gould E, Gross CG: Neurogenesis in adult mammals: some progress and problems. J Neurosci. 2002, 22: 619-623.PubMedGoogle Scholar
- Brown J, Cooper-Kuhn CM, Kempermann G, Van Praag H, Winkler J, Gage FH, et al.: Enriched environment and physical activity stimulate hippocampal but not olfactory bulb neurogenesis. Eur J Neurosci. 2003, 17: 2042-2046. 10.1046/j.1460-9568.2003.02647.x.View ArticlePubMedGoogle Scholar
- Winner B, Cooper-Kuhn CM, Aigner R, Winkler J, Kuhn HG: Long-term survival and cell death of newly generated neurons in the adult rat olfactory bulb. Eur J Neurosci. 2002, 16: 1681-1689. 10.1046/j.1460-9568.2002.02238.x.View ArticlePubMedGoogle Scholar
- Ge S, Yang CH, Hsu KS, Ming GL, Song H: A critical period for enhanced synaptic plasticity in newly generated neurons of the adult brain. Neuron. 2007, 54: 559-566. 10.1016/j.neuron.2007.05.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Michos O, Panman L, Vintersten K, Beier K, Zeller R, Zuniga A: Gremlin-mediated BMP antagonism induces the epithelial-mesenchymal feedback signaling controlling metanephric kidney and limb organogenesis. Development. 2004, 131: 3401-3410. 10.1242/dev.01251.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.